Dataset Information

Red-fleshed apples rich in anthocyanins and white-fleshed apples poor in anthocyanins modulate aorta and heart proteome in hypercholesterolemic rats. The AppleCOR study

ABSTRACT: New mechanisms-of-action of anthocyanins (ACNs) provided by a red-fleshed apple compared with a white-fleshed apple ACN-poor, and with an ACN-rich extract on the proteome profile of aorta and heart as cardiovascular key tissues were determined. Hypercholesterolemic Wistar rats were separated into the corresponding groups to analyze the proteomic profile of the aorta and heart tissues using nano-liquid chromatography coupled to mass-spectrometry. Red-fleshed apple downregulated CRP, C1QB and CFP related-inflammation. White-fleshed apple reduced C1QB, CFB, CFD, C3, and C9 related to the complement system, reduced MB and CP related to iron metabolism, and increased ME1, PKM, and PC related to energy homeostasis. ACN-rich extract increased FMOD, TAGLN, and CAP1 related to cellular structure and decreased PRKACA, IQGAP1, and HSP90AB1 related to cellular signaling. Red-fleshed apple rich in ACNs suggested an anti-inflammatory effect while white-fleshed apple reduced the complement system protein-related. An apple matrix effect reduced inflammatory proteins regardless their ACN content.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart, Aortic Root

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Pol Herrero

LAB HEAD: Nuria Canela

PROVIDER: PXD018885 | Pride | 2022-04-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	P18210-AppleCor-Plex08-02.msf	Msf
	P18210-AppleCor-Plex09-02.msf	Msf
	P18210-AppleCor-Plex10-04.msf	Msf
	P18210-AppleCor-Plex11.msf	Msf
	P18210-AppleCor-Plex12-01.msf	Msf

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Publications

Red-Fleshed Apples Rich in Anthocyanins and White-Fleshed Apples Modulate the Aorta and Heart Proteome in Hypercholesterolaemic Rats: The AppleCOR Study.

Catalán Úrsula Ú Pedret Anna A Yuste Silvia S Rubió Laura L Piñol Carme C Sandoval-Ramírez Berner Andrée BA Companys Judit J Foguet Elisabet E Herrero Pol P Canela Núria N Motilva Maria-Jose MJ Solà Rosa R

Nutrients 20220228 5

The impact of a red-fleshed apple (RFA) rich in anthocyanins (ACNs), a white-fleshed apple (WFA) without ACNs, and an extract infusion from <i>Aronia</i> fruit (AI) equivalent in dose of cyanidin-3-O-galactoside (main ACN) as RFA was determined by the proteome profile of aorta and heart as key cardiovascular tissues. Hypercholesterolaemic Wistar rats were separated into six groups (<i>n</i> = 6/group; three males and three females) and the proteomic profiles were analyzed using nanoliquid chroma ...[more]

PMID: 35268023

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