Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): T Cell, Kidney
SUBMITTER: Christina Woo
LAB HEAD: Christina Woo
PROVIDER: PXD018914 | Pride | 2022-02-15
REPOSITORIES: Pride
Items per page: 1 - 5 of 5 |
Nature chemical biology 20210308 5
O-linked N-acetylglucosamine (O-GlcNAc) is an essential and dynamic post-translational modification that is presented on thousands of nucleocytoplasmic proteins. Interrogating the role of O-GlcNAc on a single target protein is crucial, yet challenging to perform in cells. Herein, we developed a nanobody-fused split O-GlcNAcase (OGA) as an O-GlcNAc eraser for selective deglycosylation of a target protein in cells. After systematic cellular optimization, we identified a split OGA with reduced inhe ...[more]