Proteomics

Dataset Information

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Effect of HDAC inhibitors on acetylated histones in P. pacificus


ABSTRACT: Western Blot showed that epigenetic control of the mouth dimorphism in P. pacificus correlates with histone 4 acetylation. This result was validated by mass spectrometry. This experiment profiled histone acetylation in the presence and absence of the histone deacetylase (HDAC) inhibitor Trichostatin A (TSA). Histone acetylation was also examined in the presence of another HDAC inhibitor (Na-butyrate), which served as an additional negative control because unlike TSA, it did not induce a phenotypic change.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Pristionchus Pacificus

TISSUE(S): Whole Body

SUBMITTER: Nicolas Nalpas  

LAB HEAD: Ralf J. Sommer

PROVIDER: PXD018940 | Pride | 2023-02-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20190822_CO_0856RaSo_R06_1.raw Raw
20190822_CO_0856RaSo_R06_2.raw Raw
20190822_CO_0856RaSo_R06_3.raw Raw
20190822_CO_0856RaSo_R07_1.raw Raw
20190822_CO_0856RaSo_R07_2.raw Raw
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Publications

Histone 4 lysine 5/12 acetylation enables developmental plasticity of Pristionchus mouth form.

Werner Michael S MS   Loschko Tobias T   King Thomas T   Reich Shelley S   Theska Tobias T   Franz-Wachtel Mirita M   Macek Boris B   Sommer Ralf J RJ  

Nature communications 20230413 1


Development can be altered to match phenotypes with the environment, and the genetic mechanisms that direct such alternative phenotypes are beginning to be elucidated. Yet, the rules that govern environmental sensitivity vs. invariant development, and potential epigenetic memory, remain unknown. Here, we show that plasticity of nematode mouth forms is determined by histone 4 lysine 5 and 12 acetylation (H4K5/12ac). Acetylation in early larval stages provides a permissive chromatin state, which i  ...[more]

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