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Thermococcus eurythermalis A501 pH adaptation

ABSTRACT: In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of pH adaptation: low pH (pH 4) and high pH (pH 9), and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 0.1 MPa or 10 MPa) was used as the control.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Thermococcus Eurythermalis

SUBMITTER: weishu zhao

LAB HEAD: Xiang Xiao

PROVIDER: PXD018974 | Pride | 2020-09-09

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	F18FTSECKF1170_I-AFLkR004_rep1.dat	Other
	P140105_1.mgf	Mgf
	P140105_1.wiff	Wiff
	P140105_1.wiff.scan	Wiff
	P140105_10.mgf	Mgf

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Publications

Cross-Stress Adaptation in a Piezophilic and Hyperthermophilic Archaeon From Deep Sea Hydrothermal Vent.

Zhao Weishu W Ma Xiaopan X Liu Xiaoxia X Jian Huahua H Zhang Yu Y Xiao Xiang X

Frontiers in microbiology 20200910

Hyperthermophiles, living in environments above 80°C and usually coupling with multi-extreme environmental stresses, have drawn great attention due to their application potential in biotechnology and being the primitive extant forms of life. Studies on their survival and adaptation mechanisms have extended our understanding on how lives thrive under extreme conditions. During these studies, the "cross-stress" behavior in various organisms has been observed between the extreme high temperature an ...[more]

PMID: 33013758

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Thermococcus eurythermalis A501, Temperature adaptation

Project description:In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of temperature adaptation: low temperature (65°C) and high temperature (95°C), and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 0.1 MPa or 10 MPa) was used as the control.

2020-09-09 | PXD018991 | Pride

Thermococcus eurythermalis A501, high hydrostatic pressure

Project description:In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of pressure adaptation: HP (40 MPa) tested under 85°C and 95°C, and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 10 MPa) was used as the control.

2020-09-09 | PXD019007 | Pride

Thermococcus eurythermalis A501, NaCl adaptation

Project description:In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of NaCl adaptation: low salinity (1.5% NaCl, w/v) and high salinity (4.5% NaCl, w/v), and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 0.1 MPa or 10 MPa) was used as the control.

2020-09-09 | PXD018994 | Pride

Ctenopharyngodon idella iTRAQ - Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

Project description:Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

2018-12-18 | PXD012011 | Pride

myocardial tissue from atrial fibrillation patients

Project description:Electrical and structural remodeling processes are contributors to the self-perpetuating nature of atrial fibrillation (AF). However, their correlation has not been clarified. In this study, human atrial tissues from the patients with rheumatic mitral valve disease in either sinus rhythm or persistent AF were analyzed using a combined transcriptomic and proteomic approach. An up-regulation in chloride intracellular channel (CLIC) 1, 4, 5 and a rise in type IV collagen were revealed. Combined with the results from immunohistochemistry and electron microscope analysis, the distribution of type IV collagen and effects of fibrosis on myocyte membrane indicated the possible interaction between CLIC and type IV collagen, confirmed by protein structure prediction and co-immunoprecipitation. These results indicate that CLICs play an important role in the development of atrial fibrillation and that CLICs and structural type IV collagen may interact on each other to promote the development of AF in rheumatic mitral valve disease.

2018-11-22 | PXD006911 | Pride

Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology

Project description:Transplantation is currently the best treatment option for end-stage renal disease (ESRD) patients. However, acute rejection (AR) is the main source of failure in renal transplantation. The current gold standard for diagnosis of AR involves renal biopsy, but it is invasive, time consuming, costly and inconvenient. Sensitive and less invasive detection of AR episodes in renal transplant patients is essential to preserve the allograft function. Here, we applied isobaric tags for relative and absolute quantisation (iTRAQ) mass spectrometry to analyse serum protein expression in AR patients and healthy controls. Overall, 1399 proteins were identified. Using a cut-off of Q<0.05 and a fold change > 1.2 for the expressed variation, 109 proteins showed distinct differential expression between the AR and control groups, of whom 72 were upregulated and 37 were downregulated. Several proteins, such as properdin, keratin 1, lipoprotein (a) and vitamin D-binding protein, may play roles in the pathogenesis of AR. This study focused on iTRAQ-based proteomic profiling of serum samples in AR. Insights from our work might help advance our understanding of the molecular mechanisms of AR and identify potential novel biomarkers of AR for further characterization.

2021-09-08 | PXD015336 | Pride

The proteomics analysis of Crassostrea gigas under Zn and Cd contamination

Project description:Understanding organisms’ environmental adaptation mechanisms is a fundamental question in ecology and evolution. The oyster Crassostrea gigas is able to accumulate high levels of zinc (Zn) and cadmium (Cd) without self-poisoning, and is thus widely used for pollution detection. However, the molecular mechanisms underlying such stress adaptation are largely unknown. Therefore, this study aimed to provide fundamental insights into the complex Zn/Cd-response regulatory networks.

2018-10-25 | PXD003290 | Pride

Human dermal papilla cell culture medium iTRAQ

Project description:Alopecia is an exceedingly prevalent problem and lacks effective therapy. Recently, research has focused on early-passage dermal papilla cells (DPCs), which have hair inducing activity both in vivo and in vitro. Our previous study indicated that factors secreted from early-passage DPCs contribute to hair follicle (HF) regeneration. To identify which factors are responsible for HF regeneration and why late-passage DPCs lose this potential，we collected 48-h-culture medium (CM) from both of passage 3 and 9 DPCs and subcutaneously injected the DPC-CM into NU/NU mice. Passage 3 DPC-CM induced HF regeneration, based on the emergence of a white hair coat, but passage 9 DPC-CM not. In order to identify the key factors responsible for hair inductive capacity, CM from passage 3 and 9 DPCs was analyzed by iTRAQ-based quantitative proteomic technology. We identified 1360 proteins, of which 213 proteins were differentially expressed between CM from early-passage vs. late-passage DPCs, including SDF1, MMP3, biglycan and LTBP1.Further analysis indicated that the differentially-expressed proteins regulated the Wnt, TGF-β and BMP signaling pathways, which directly and indirectly participate in HF morphogenesis and regeneration. Subsequently, we selected 19 proteins for further verification by multiple reaction monitoring (MRM) between the two types of CM. These results would be used to identify the key factors for inducing HF regeneration and reveal the molecular mechanisms of losing inductive ability of DPCs. Furthermore, it is possible to explore some drugs for alopecia in the clinic according to this secretome date analysis.

2018-10-26 | PXD004201 | Pride

Proteomic analysis of the immune response to dsRNA in B.dorsalis

Project description:Proteomic analysis of a mixed sample from 8, 12 and 24 h (early set) post first exposure to dsRNA and a sample from 5 days (late set) post first exposure to dsRNA to identify differentially expressed proteins which were responsible to the immune response to dsRNA in B.dorsalis.

2018-10-27 | PXD004272 | Pride

Cotton fiber LC-MS/MS - Proteomic Analysis of Differences in Fiber Development between Wild and Cultivated Gossypium hirsutum L

Project description:Upland cotton (Gossypium hirsutum L.) is one of the world’s most important fiber crops, accounting for more than 90% of all cotton production. While their wild progenitors have relatively short and coarse, often tan-colored fibers, modern cotton cultivars possess longer, finer, stronger, and whiter fiber. In this study, the wild and cultivated cottons (YU-3 and TM-1) selected show significant differences on fibers at 10 day post-anthesis (DPA), 20 DPA and mature stages at the physiological level. In order to explore the effects of domestication, reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality, an iTRAQ-facilitated proteomic methods were performed on developing fibers. There were 6990 proteins identified, among them 336 were defined as differentially expressed proteins (DEPs) between fibers of wild versus domesticated cotton. The down- or up-regulated proteins in wild cotton were involved in Phenylpropanoid biosynthesis, Zeatin biosynthesis, Fatty acid elongation and other processes. Association analysis between transcroptome and proteome showed positive correlations between transcripts and proteins at both 10 DPA and 20 DPA. The difference of proteomics had been verified at the mRNA level by qPCR, also at physiological and biochemical level by POD activity determination and ZA content estimation. This work corroborate the major pathways involved in cotton fiber development and demonstrate that POD activity and zeatin content have a great potential related to fiber elongation and thickening.

2017-08-28 | PXD006604 | Pride

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