Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of RNG140 (caprin2)-associated proteins in CHO cells

ABSTRACT: Regulation of gene expression at the translational level is key to determining cell fate and function. An RNA-binding protein RNG140 (caprin2) plays a role in cell differentiation such as eye lens development and has been reported to function in translational regulation. To identify RNG140-associated proteins, we generated CHO cells stably expressing GFP and RNG140-GFP, and performed immunoprecipitation (IP) with GFP antibody from those cells followed by mass spectrometry. We identified about 400 proteins specifically co-immunoprecipitated with RNG140-GFP but not with GFP. Gene ontology (GO) enrichment analysis revealed that the eIF3 subunit proteins and small ribosomal subunit proteins were significantly enriched in the RNG140-associated complex.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Epithelial Cell

SUBMITTER: Kaori Nakazawa

LAB HEAD: Nobuyuki Shiina

PROVIDER: PXD019040 | Pride | 2020-08-17

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	GFP.xlsx	Xlsx
	GFP1.raw	Raw
	GFP10.raw	Raw
	GFP11.raw	Raw
	GFP12.raw	Raw

Items per page:

1 - 5 of 44

Similar Datasets

RNA-sequencing analysis of a wildtype CHO cell line (CHO-K1), a mutant CHO cell line (pgsA745) that lacks xylosyltransferase (XylT) activity, and pgsA745 stably transfected with XylT1 (pgsA745-XylT1).

Project description:We report a a large deletion in the gene encoding for laminin subunit α2 (Lama2) in CHO pgsA745.

2016-11-20 | GSE75521 | GEO

eIF3 PAR-CLIP in 293T cells

Project description:Regulation of protein synthesis is fundamental for all aspects of eukaryotic biology by controlling development, homeostasis, and stress responses. The 13-subunit, 800-kDa eukaryotic initiation factor 3 (eIF3) organizes initiation factor and ribosome interactions required for productive translation. However, current understanding of eIF3 function does not explain genetic evidence correlating eIF3 deregulation with tissue-specific cancers and developmental defects. Here we report the genome-wide discovery of human transcripts that interact with eIF3 using photo-activatable crosslinking and immunoprecipitation (PAR-CLIP). eIF3 binds to a highly specific programme of messenger RNAs (mRNAs) involved in cell growth control processes, including cell cycling, differentiation, and apoptosis, via the mRNA 5' untranslated region (5' UTR). Surprisingly, functional analysis of the interaction between eIF3 and two mRNAs encoding cell proliferation regulators, c-Jun and BTG1, reveals that eIF3 employs different modes of RNA stem loop binding to exert either translational activation or repression. Our findings illuminate a new role for eIF3 in governing a specialized repertoire of gene expression and suggest that binding of eIF3 to specific mRNAs could be targeted to control carcinogenesis. 293T cells were treated with 4-thiouridine and protein-RNA complexes were crosslinked, and eIF3-RNA complexes were immunoprecipitated.

2015-04-06 | E-GEOD-65004 | biostudies-arrayexpress

Ribosome profiling-guided depletion of an mRNA improves CHO cell growth and recombinant product titers

Project description:We present the first comprehensive genome-wide view of CHO translational activity during recombinant expression by the application of ribosome profiling. The distribution of translational power in CHO cells was analyzed in the context of the recombinant mRNAs and the endogenous mRNA pool. In an antibody-producing CHO cell line, the recombinant mRNAs were found to be the most abundant transcripts and also sequestered a substantial amount of translating ribosomes (up to 15%). The recombinant mRNAs were translated as efficiently as the endogenous mRNAs, and changes in translation and transcription of the recombinant mRNAs were directly reflected in changes in specific productivity. Interestingly, improvements in bioprocess quality attributes were achieved by depleting the highly expressed and translated inert NeoR mRNA by siRNA-mediated knock-down. This resulted in improved cellular growth, which was accompanied by an 18% increase in antibody titers. This study is the first to carefully map the translatome of the CHO cell to the nucleotide level and to demonstrate how recombinant expression affects the cell on the translational level.

2016-12-21 | GSE79512 | GEO

CHO-S transcriptome in batch culture

Project description:Transcriptomic data from CHO-S cells were gathered from multiple time points during batch culture to provide information about gene presence/absence for generation of a CHO-S cell line specific model using the GIMME algorithm.

2016-11-23 | GSE77800 | GEO

Cricetulus griseus strain:CHO

Project description:CHO-DG44

| PRJDB5831 | ENA

CHO cells: Serum free adapted cells (Control) vs. Serum adapted cells

Project description:Transcriptional profiling of CHO-K1 cells comparing to in-house serum-free and suspension adapted CHO-K1 cells in the exponential phase. Goal was to determine the effects of serum on CHO-K1 cells.

2016-12-24 | GSE92885 | GEO

Cricetulus griseus strain:CHO-K1

Project description:CHO cells

| PRJDB4351 | ENA

Identification of microRNAs specific for high producer CHO cell lines

Project description:Mature microRNA levels were analyzed in 4 producing CHO cell lines expressing 2 structurally different recombinant proteins at low and high level as well as in 1 non-producing CHO cell line. The goal was to identify microRNAs that are involved in heterologous protein formation and secretion. 5 recombinant CHO cell lines. 3 biological replicates each. All samples were hybridized against a common reference (pool of all total RNA samples).

2014-08-04 | E-GEOD-57023 | biostudies-arrayexpress

eIF3 PAR-CLIP in 293T cells

2015-04-06 | GSE65004 | GEO

Analysis of mature miRNA levels in the context of CHO cell proliferation

Project description:Mature microRNA transcripts were analyzed in 9 CHO cell lines growing at different proliferation rates. The goal was to identify microRNAs that correlate positively or negatively with CHO proliferation rates.

2015-12-10 | GSE75830 | GEO