Proteomics

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Acute effects of Salbutamol in Mouse Kidney Distal Convoluted Tubules (mpkDCT) Cells

ABSTRACT: The sympathetic nervous system (SNS) plays a central role in blood pressure regulation. Recent studies have shown that cells of the distal convoluted tubule (DCT) can be stimulated directly via the beta-adrenergic receptor resulting in activation of the NaCl cotransporter NCC. Whether these effects are mediated by the beta1- or beta2-adrenergic receptor is unclear, and the acute signaling cascades rapidly activated by beta-adrenergic receptor stimulation in the DCT are unknown. Here in this study, we aim to identify the rapid salbutamol-mediated (beta2-adrenergic receptor) signaling in the DCT by looking at global protein phosphorylation changes in the mpkDCT cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Kidney

SUBMITTER: Lei Cheng  

LAB HEAD: Robert A. Fenton

PROVIDER: PXD019051 | Pride | 2021-05-17

REPOSITORIES: Pride

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Activation of the kidney sodium chloride cotransporter by the β2-adrenergic receptor agonist salbutamol increases blood pressure.

Poulsen Søren B SB   Cheng Lei L   Penton David D   Kortenoeven Marleen L A MLA   Matchkov Vladimir V VV   Loffing Johannes J   Little Robert R   Murali Sathish K SK   Fenton Robert A RA  

Kidney international 20210430 2

The thiazide-sensitive sodium-chloride-cotransporter (NCC) in the kidney distal convoluted tubule (DCT) plays an essential role in sodium and potassium homeostasis. Here, we demonstrate that NCC activity is increased by the β2-adrenoceptor agonist salbutamol, a drug prevalently used to treat asthma. Relative to β1-adrenergic receptors, the β2-adrenergic receptors were greatly enriched in mouse DCT cells. In mice, administration of salbutamol increased NCC phosphorylation (indicating increased ac  ...[more]

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