Proteomics

Dataset Information

0

EDEM1 interaction proteomics in HEK293T cells

ABSTRACT: Misfolded or unfolded proteins from the endoplasmic reticulum (ER) are sent to proteasomal degradation via the Endoplasmic Reticulum Associated Degradation (ERAD) pathway. We provide here a proteomic analysis of the interaction partners for one of the ER mannosidases involved in ERAD, ER degradation-enhancing alpha-mannosidase-like protein 1 (EDEM1) and its mutant, a previously described version of EDEM, lacking its N-terminal disordered region (Δ-EDEM1). Moreover, we also performed a whole proteome analysis for cells overexpressing both, WT and Δ-EDEM1 and investigated the proteins which co-fractionate in a sucrose gradient for both EDEM1 and Δ-EDEM1 and their associated proteins.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Cristian Munteanu  

LAB HEAD: Ștefana M. Petrescu

PROVIDER: PXD019066 | Pride | 2020-05-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
01HEK-E1_WT_PBS-T_36-52kDa_run01.raw Raw
01HEK-E1_WT_PBS-T_36-52kDa_run02.raw Raw
01HEK-E1_WT_PBS-T_36-52kDa_run03.raw Raw
01HEK-E1_WT_PBS-T_52-115kDa_run01.raw Raw
01HEK-E1_WT_PBS-T_52-115kDa_run02.raw Raw
Items per page:
1 - 5 of 230
altmetric image

Publications

EDEM1 Drives Misfolded Protein Degradation via ERAD and Exploits ER-Phagy as Back-Up Mechanism When ERAD Is Impaired.

Chiritoiu Marioara M   Chiritoiu Gabriela N GN   Munteanu Cristian V A CVA   Pastrama Florin F   Ivessa N Erwin NE   Petrescu Stefana M SM  

International journal of molecular sciences 20200514 10

Endoplasmic reticulum (ER)-associated degradation (ERAD) is the main mechanism of targeting ER proteins for degradation to maintain homeostasis, and perturbations of ERAD lead to pathological conditions. ER-degradation enhancing α-mannosidase-like (EDEM1) was proposed to extract terminally misfolded proteins from the calnexin folding cycle and target them for degradation by ERAD. Here, using mass-spectrometry and biochemical methods, we show that EDEM1 is found in auto-regulatory complexes with  ...[more]

Similar Datasets

Proteomics Human EDEM2 proteomics and glycoproteomics datasets
2021-07-30 | PXD025881 | Pride
Proteomics Human EDEM2 proteomics and glycoproteomics datasets
2022-04-13 | PXD022566 | Pride
Transcriptomics IRE1alpha is an endogenous substrate of endoplasmic reticulum-associated degradation
2015-11-04 | E-GEOD-70563 | biostudies-arrayexpress
Transcriptomics Transient expression of misfolded surfactant protein C
2009-05-14 | E-GEOD-16054 | biostudies-arrayexpress
Transcriptomics Regulation of endoplasmic reticulum-mitochondria contacts and mitochondrial dynamics by Sel1L-Hrd1 ERAD during thermogenesis
2020-04-24 | GSE145895 | GEO
Transcriptomics IRE1alpha is an endogenous substrate of endoplasmic reticulum-associated degradation
2015-11-04 | GSE70563 | GEO
Transcriptomics Transcriptomic responses to SEL1L-HRD1 ERAD deficiency in HEK293T cells and mouse brown adipose tissue
2023-12-17 | GSE231583 | GEO
Transcriptomics Sel1L is Indispensable for Mammalian ERAD, ER Homeostasis and Survival
2013-12-04 | E-GEOD-52929 | biostudies-arrayexpress
Proteomics Early Secretory Pathway glycoprotein analysis dataset
2023-01-31 | PXD035541 | Pride
Transcriptomics ER-associated degradation maintains postnatal muscle hypertophy and systemic energy metabolism
2023-07-17 | GSE237194 | GEO