Dataset Information

Proteomic profile of nucleoid-associated proteins isolated from Mycoplasma gallisepticum in different growth phases

ABSTRACT: Mycoplasma gallisepticum (MG) is one of the smallest free-living and self-replicating organisms, it is characterized by lack of cell wall and reduced genome size. As a result of genome reduction, MG has a limited variety of DNA-binding proteins (DBP) and transcription factors. To investigate the dynamic changes of the proteomic profile of MG nucleoid, that may assist in revealing its mechanisms of functioning, regulation of chromosome organization and stress adaptation, a quantitative proteomic study was performed on MG nucleoids obtained from the cell culture in logarithmic and stationary phases of synchronous growth. MG cells were grown in a liquid medium with a 9h starvation period. Nucleoids were obtained from the cell culture at the 26th and the 50th hour (logarithmic and stationary phases respectively) by sucrose density gradient centrifugation. LC-MS analysis was carried out on an Ultimate 3000 RSLCnano HPLC system connected to a Fusion Lumos mass spectrometer, controlled by XCalibur software (ThermoFisher Scientific) via a nanoelectrospray source (Thermo Fisher Scientific). For comprehensive peptide library generation one sample from each biological replicate was run in DDA mode. Then, all the sample were run in a single LC-MS DIA run. Identification of DDA files and DIA quantitation was performed with MaxQuant and Skyline software.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mycoplasma Gallisepticum S6

SUBMITTER: Aleksandr Zubov

LAB HEAD: Gleb Fisunov

PROVIDER: PXD019077 | Pride | 2020-05-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DIA_method.pdf	Pdf
	DIA_nucleoids_02052020.sky	Other
	DIA_nucleoids_02052020.sky.view	Other
	DIA_nucleoids_02052020.skyd	Other
	DIA_nucleoids_02052020.skyl	Other

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Data on nucleoid-associated proteins isolated from <i>Mycoplasma Gallisepticum</i> in different growth phases.

Zubov Aleksandr I I Semashko Tatiana A A Evsyutina Daria V V Ladygina Valentina G G Kovalchuk Sergey I I Ziganshin Rustam H H Galyamina Maria A A Fisunov Gleb Yu Y Pobeguts Olga V V

Data in brief 20200612

<i>Mycoplasma gallisepticum</i> (MG) is one of the smallest free-living and self-replicating organisms, it is characterized by lack of cell wall and reduced genome size. As a result of genome reduction, MG has a limited variety of DNA-binding proteins and transcription factors. To investigate the dynamic changes of the proteomic profile of MG nucleoid, that may assist in revealing its mechanisms of functioning, regulation of chromosome organization and stress adaptation, a quantitative proteomic ...[more]

PMID: 32637477

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