Dataset Information

Equine gluteal muscle LC-MSMS: Warmblood

ABSTRACT: Myofibrillar myopathy (MFM) in horses is a late onset disease that affects performance and athleticism. It is characterized by myofibrillar disarray and protein aggregation with no known cause. The objective of this study was to elucidate the molecular drivers of MFM in Warmblood (WB) horses by proteomic profiling (5 MFM WB, 4 non-MFM WB) of gluteal muscle. MFM horses used in this study had a chronic history of poor performance and exercise intolerance as well as accumulation of desmin aggregates in > 4 myofibers per muscle sample. The Equine Neuromuscular Diagnostic Laboratory database at Michigan State University was queried to identify WB horses with snap frozen gluteus medius biopsies available for analysis. Non-MFM control horses were defined as horses with no history of exercise intolerance and no evidence of desmin accumulation or other histopathology in muscle biopsies. Muscle biopsy samples were obtained at rest from horses that had not undertaken strenuous exercise in the preceding 48 hours.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Skeletal Muscle Fiber, Skeletal Muscle

DISEASE(S): Myofibrillar Myopathy

SUBMITTER: Stephanie Valberg

LAB HEAD: Stephanie Valberg

PROVIDER: PXD019187 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ScaffoldQuantMLReportforWB_proteomics_merged_20180906.sqml	Other
	UP000002281-GCA_002863925.1-cRAP.fasta	Fasta
	WB_proteomics_merged_20180906.mzid.gz	Mzid
	WB_proteomics_merged_20180906.mzid_Mudpit_2018_5_13_40_PM_File_Size__703527597__Byte__(F010569).pride.mgf.gz	Mzid
	WB_proteomics_merged_20180906.mzid_Mudpit_2018_5_13_40_PM_File_Size__703527597__Byte__F010569.MGF	Mzid

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Integrated proteomic and transcriptomic profiling identifies aberrant gene and protein expression in the sarcomere, mitochondrial complex I, and the extracellular matrix in Warmblood horses with myofibrillar myopathy.

Williams Zoë J ZJ Velez-Irizarry Deborah D Gardner Keri K Valberg Stephanie J SJ

BMC genomics 20210611 1

<h4>Background</h4>Myofibrillar myopathy in humans causes protein aggregation, degeneration, and weakness of skeletal muscle. In horses, myofibrillar myopathy is a late-onset disease of unknown origin characterized by poor performance, atrophy, myofibrillar disarray, and desmin aggregation in skeletal muscle. This study evaluated molecular and ultrastructural signatures of myofibrillar myopathy in Warmblood horses through gluteal muscle tandem-mass-tag quantitative proteomics (5 affected, 4 cont ...[more]

PMID: 34112090

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