Dataset Information

Urine Large Extracellular Vesicle LC-MS/MS Data from a Chronic Kidney Disease Rat Model

ABSTRACT: This study examined large renal tubule extracellular vesicles (LRT-EVs) isolated from urine of a 5/6 nephrectomy (5/6Nx) rat model that recapitulates many elements of human chronic kidney disease (CKD). Within weeks of 5/6Nx rats spontaneously exhibit proximal tubular damage including brush boarder shedding and the production of LRT-EVs. Analysis of the LRT-EV size and a lack of TUNEL staining in 5/6Nx rats, suggested LRT-EVs to be distinct from exosomes, microvesicles, and apoptotic bodies.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Urine, Extracellular Vesicle

DISEASE(S): Chronic Kidney Disease

SUBMITTER: Brian Hoffmann

LAB HEAD: Alison Kriegel, PhD

PROVIDER: PXD019207 | Pride | 2021-07-26

REPOSITORIES: Pride

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Functionally Essential Tubular Proteins Are Lost to Urine-Excreted, Large Extracellular Vesicles during Chronic Renal Insufficiency.

Adam Ryan J RJ Paterson Mark R MR Wardecke Lukus L Hoffmann Brian R BR Kriegel Alison J AJ

Kidney360 20201029 10

<h4>Background</h4>The 5/6 nephrectomy (5/6Nx) rat model recapitulates many elements of human CKD. Within weeks of surgery, 5/6Nx rats spontaneously exhibit proximal tubular damage, including the production of very large extracellular vesicles and brush border shedding. We hypothesized that production and elimination of these structures, termed large renal tubular extracellular vesicles (LRT-EVs), into the urine represents a pathologic mechanism by which essential tubule proteins are lost.<h4>Me ...[more]

PMID: 34263177

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