Dataset Information

Proteome changes in monocytic THP-1 cells induced by 31 engineered nanomaterials

ABSTRACT: Here, we investigated a panel of 31 engineered nanomaterials (ENMs) with different core chemistries and surface modifications using conventional cytotoxicity assays coupled with omics-based approaches. Proteomics analysis were conducted in order to monitor changes in cells proteome exposed to ENMs. In this standard approach, cells are exposed to various types and amounts of ENMs and proteins are extracted after a given exposure period. For controls, no ENM are applied but the incubation and extraction of proteins is done the same way. Proteome changes are monitored using high-resolution Fourier transform mass spectrometry (Orbitraps) as well as in-house developed label-free software. Similar approaches have been used for quantitative analysis of posttranslational modifications. Proteomics analyses following low-dose exposure of THP-1 cells suggested a non-specific stress response to ENMs while microarray-based profiling revealed significant changes in gene expression as a function of both surface functionalization and core chemistry of the ENMs. Pathway analysis revealed that the most biologically active ENMs displaying cationic surfaces downregulated DNA replication/cell cycle responses and upregulated inflammatory responses. This study shows that surface chemistry is a key determinant of nanomaterial effects on immune cells.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte

SUBMITTER: Jimmy Ytterberg

LAB HEAD: Prof. Roman Zubarev

PROVIDER: PXD019267 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20151217_001_Qp2_78_PROT_1_Au54_24_EC10_3_.raw	Raw
	20151217_002_Qp2_27_PROT_1_CNT4_24_EC10_3_.raw	Raw
	20151217_003_Qp2_94_PROT_1_ND4_24_EC10_1_.raw	Raw
	20151217_004_Qp2_41_PROT_1_TiO2r1_24_EC10_2_.raw	Raw
	20151217_005_Qp2_21_PROT_1_CNT2_24_EC10_3_.raw	Raw

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Publications

Multiparametric Profiling of Engineered Nanomaterials: Unmasking the Surface Coating Effect.

Gallud Audrey A Delaval Mathilde M Kinaret Pia P Marwah Veer Singh VS Fortino Vittorio V Ytterberg Jimmy J Zubarev Roman R Skoog Tiina T Kere Juha J Correia Manuel M Loeschner Katrin K Al-Ahmady Zahraa Z Kostarelos Kostas K Ruiz Jaime J Astruc Didier D Monopoli Marco M Handy Richard R Moya Sergio S Savolainen Kai K Alenius Harri H Greco Dario D Fadeel Bengt B

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20201011 22

Despite considerable efforts, the properties that drive the cytotoxicity of engineered nanomaterials (ENMs) remain poorly understood. Here, the authors inverstigate a panel of 31 ENMs with different core chemistries and a variety of surface modifications using conventional in vitro assays coupled with omics-based approaches. Cytotoxicity screening and multiplex-based cytokine profiling reveals a good concordance between primary human monocyte-derived macrophages and the human monocyte-like cell ...[more]

PMID: 33240770

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