Dataset Information

Subclinical effects of remote ischaemic conditioning in human kidney transplants revealed by quantitative proteomics

ABSTRACT: Background: Remote ischaemic conditioning (RIC) is currently being explored as a non-invasive method to attenuate ischaemia/reperfusion injuries in organs. A randomised clinical study (CONTEXT) evaluated the effects of RIC compared to non-RIC controls in human kidney transplants. Methods: RIC was induced prior to kidney reperfusion by episodes of obstruction to arterial flow in the l eg opposite the transplant using a tourniquet (4 x 5min). Although RIC did not lead to clinical improvement of transplant outcomes, we explored whether RIC induced molecular changes through precision analysis of CONTEXT recipient plasma and kidney tissue samples by high-resolution tandem mass spectrometry (MS/MS). Results: We observed an accumulation of muscle derived proteins in kidney tissue proteomes and altered mitochondrial proteins, likely provoked by RIC, which was not reflected in plasma. In addition, MS/MS analysis demonstrated transient upregulation of several acute phase response proteins (SAA1, SAA2, CRP) in plasma, 1 and 5 days post-transplant. RIC showed a lower magnitude change when compared to non-RIC. However, RIC did not have a consistent effect on the magnitude of acute inflammation following clinical intervention. Conclusions: Together, our results indicate sub-clinical systemic and organ-localised effects of RIC.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Kidney

SUBMITTER: Darragh O'Brien

LAB HEAD: Benedikt M Kessler

PROVIDER: PXD019284 | Pride | 2021-09-09

REPOSITORIES: Pride

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	HF361_MSQ1381_20180615_Honglei_s10_180629164949.raw	Raw
	HF361_MSQ1381_20180615_Honglei_s11_180629192809.raw	Raw
	HF361_MSQ1381_20180615_Honglei_s12_180629220632.raw	Raw
	HF361_MSQ1381_20180615_Honglei_s13_180630004452.raw	Raw
	HF361_MSQ1381_20180615_Honglei_s14_180630032319.raw	Raw

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Subclinical effects of remote ischaemic conditioning in human kidney transplants revealed by quantitative proteomics.

Thorne Adam M AM Huang Honglei H O'Brien Darragh P DP Eijken Marco M Krogstrup Nicoline Valentina NV Norregaard Rikke R Møller Bjarne B Ploeg Rutger J RJ Jespersen Bente B Kessler Benedikt M BM

Clinical proteomics 20201102 1

<h4>Background</h4>Remote ischaemic conditioning (RIC) is currently being explored as a non-invasive method to attenuate ischaemia/reperfusion injuries in organs. A randomised clinical study (CONTEXT) evaluated the effects of RIC compared to non-RIC controls in human kidney transplants.<h4>Methods</h4>RIC was induced prior to kidney reperfusion by episodes of obstruction to arterial flow in the leg opposite the transplant using a tourniquet (4 × 5 min). Although RIC did not lead to clinical impr ...[more]

PMID: 33292164

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