Dataset Information

Quantitative Proteomics comparison of total expressed proteomes of Anisakis simplex sensu stricto, A. pegreffii and their hybrid genotype

ABSTRACT: The total proteomes of Anisakis simplex s.s., A. pegreffii and their hybrid genotype have been compared by quantitative proteomics (iTRAQ approach), which considers the level of expressed proteins. A total of 1,976 proteins have been identified using public databases. One hundred ninety six proteins were found significantly differentially expressed; results of pairwise Log2 ratio comparisons among them were statistically treated and supported in order to convert them into discrete character states. This comparison selected thirty six proteins as discriminant biomarkers among A. simplex, A. pegreffii and their hybrid genotype; eighteen of these biomarkers, encoded by nine loci, are specific allergens of Anisakis (Ani s7, Ani s8, Ani s12 and Ani s14) and other (Ancylostoma secreted) is a common nematodes venom allergen.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Anisakis Simplex Complex

SUBMITTER: Sergio Ciordia

LAB HEAD: Alfonso Navas

PROVIDER: PXD019289 | Pride | 2020-08-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20160920_01_ANavas-Anisakis_iTRAQ-Rep1.wiff.zip	Wiff
	20160920_03_ANavas-Anisakis_iTRAQ-Rep2.wiff.zip	Wiff
	20160920_05_ANavas-Anisakis_iTRAQ-Rep3.wiff.zip	Wiff
	20170123_07_Anavas_iTRAQ3-Rep1.wiff.zip	Wiff
	20170123_09_Anavas_iTRAQ3-Rep2.wiff.zip	Wiff

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Publications

Quantitative Proteomics Comparison of Total Expressed Proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and Their Hybrid Genotype.

C Arcos Susana S Robertson Lee L Ciordia Sergio S Sánchez-Alonso Isabel I Careche Mercedes M Carballeda-Sanguiao Noelia N Gonzalez-Muñoz Miguel M Navas Alfonso A

Genes 20200810 8

The total proteomes of Anisakis simplex s.s., A. pegreffii and their hybrid genotype have been compared by quantitative proteomics (iTRAQ approach), which considers the level of expressed proteins. Comparison was made by means of two independent experiments considering four biological replicates of A. simplex and two each for A. pegreffii and hybrid between both species. A total of 1811 and 1976 proteins have been respectively identified in the experiments using publi ...[more]

PMID: 32785065

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Project description:Fruit ripening is a very important physiological process which gains relevance in crop species due to their economical and nutritional repercussions. During fruit ripening enormous metabolic changes occur in a genetically-controlled scenario affecting the physiology of most cell compartments. Peroxisomes are single-membrane bounded organelles present in all eukaryotes which display a noteworthy nitro-oxidative metabolism, and harbor catalase as one of the major antioxidant enzymes in cells. Quantitative proteomics analysis by isobaric tags for relative and absolute quantification (iTRAQ) was used to investigate the ripening process in sweet pepper (Capsicum annuum L.) fruits. Out of 2,574 quantified proteins, 692 were found to be significantly more abundant in immature green fruits compared to red ones, but 497 showed a lower expression as the ripening proceeded. Overall, about 50% of the detectable proteins were estimated to modify their expression due to the ripening process. Data obtained from the Gene Ontology algorithms (AgriGO platform) showed that from all the identified proteins, 46 (2%) were only predicted to have a peroxisomal origin, with a high number of them displaying up-expression tendency during ripening. Catalase was framed within the group which showed lower expression in ripe fruits, but this enzyme was also susceptible to undergo posttranslational modifications (PTMs) promoted by reactive nitrogen and oxygen species (RNS and ROS) through nitration, S-nitrosylation and oxidation events which provoked its inhibition. The biochemical characterization of catalase indicates that it has atypical native molecular mass (125-135 kDa), since it behaves as a homodimer, and isoelectric point of 7.4, which is higher than that of the majority of plant catalases reported so far. Taking together, these data suggest that ROS and RNS could be essential to modulate the role of catalase for the maintenance of basic cellular peroxisomal functions during pepper fruit ripening where nitro-oxidative stress occur.

Dataset Information

Quantitative Proteomics comparison of total expressed proteomes of Anisakis simplex sensu stricto, A. pegreffii and their hybrid genotype

Dataset's files

Publications

Quantitative Proteomics Comparison of Total Expressed Proteomes of <i>Anisakis simplex</i> Sensu Stricto, <i>A. pegreffii</i>, and Their Hybrid Genotype.

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