Dataset Information

Ozone-derived oxisterols impair lung macrophage phagocytosis via adduction of phagocytosis receptors

ABSTRACT: Inhalation of the amibient air polution ozone causes lung inflammation and can suppress host defense mechanisms, including impairing macrophage phagocytosis. Ozone reacts with cholesterol in the lung to form oxysterols, like secosterol A and secosterol B, which can form covalent adducts on cellular proteins. How oxysterol-protein adduction modifies the function of lung macrophages is unknown. Herein, we used a preoteomic screen to identify lung macrophage proteins that fomr adducts with ozone-derived oxysterols. Analysis show that the phagocytic receptor CD206 and CD64 formed adducts with secosterol A. Adduction of these receptors with ozone-derived oxysterols impaired ligand binding and corresponded with reduced macrophage phagocytosis. This work suggests a novle mechanism for the suppression of macrophage phagocytosis following ozone exposure through the generation of oxysterols and the formation of oxysterol-protein adducts on phagocytic receptors.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Alveolar Macrophage

SUBMITTER: Hye-Young Kim

LAB HEAD: Ilona, Jaspers

PROVIDER: PXD019317 | Pride | 2020-07-18

REPOSITORIES: Pride

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			Action	DRS
	sh_5282_HYK_100918_Mac_aSA_100mM.mzML	Mzml
	sh_5282_HYK_100918_Mac_aSA_100mM.mzid.gz	Mzid
	sh_5282_HYK_100918_Mac_aSA_100mM.pride.mgf.gz	Mgf
	sh_5282_HYK_100918_Mac_aSA_100mM.pride.mztab.gz	Mztab
	sh_5282_HYK_100918_Mac_aSA_100mM.raw	Raw

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Ozone-derived oxysterols impair lung macrophage phagocytosis via adduction of some phagocytosis receptors.

Duffney Parker F PF Kim Hye-Young H HH Porter Ned A NA Jaspers Ilona I

The Journal of biological chemistry 20200720 36

Inhalation of the ambient air pollutant ozone causes lung inflammation and can suppress host defense mechanisms, including impairing macrophage phagocytosis. Ozone reacts with cholesterol in the lung to form oxysterols, like secosterol A and secosterol B (SecoA and SecoB), which can form covalent adducts on cellular proteins. How oxysterol-protein adduction modifies the function of lung macrophages is unknown. Herein, we used a proteomic screen to identify lung macrophage proteins that form addu ...[more]

PMID: 32690608

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