Dataset Information

Atherosclerotic environment effect over CAC pre-angiogenic role

ABSTRACT: In response to vascular injury, early EPC or Circulating angiogenic cells (CAC) are thought to be recruited to the damaged areas, participating mainly in a paracrine fashion, by releasing pro-angiogenic factors, promoting the mobilization of other cell populations such as the late EPC, or endothelial colony-forming cells (ECFC). ECFC will help by replacing the damaged endothelium and promoting neovascularization. Unfortunately, however, despite the regenerative role, the number and function of EPC are severely affected under pathological conditions, making compulsory to better understand how these cells react under those environments in order to implement their use in regenerative cell therapies. Herein we evaluated, for the first time to our knowledge, the effect of atherosclerotic factors over the paracrine role of CAC, by direct incubation ex vivo of healthy CAC with the secretome of atherosclerotic arteries. The application of a label free proteomics approach allowed the identification of 194 altered proteins in the secretome of pre-conditioned CAC, many of them related, among others, with inhibition of angiogenesis and cell migration. Functional assays corroborated that, after atherosclerotic pre-conditioning, the pro-angiogenic effect of CAC over ECFC was impaired, affecting ECFC migration as well as their ability to form tubules on a basement membrane matrix assay. Up-regulation of several anti-angiogenic proteins in the CAC secretome could help to explain such angiogenic switch which, in turns, could reflect a protective and beneficial effect against the atherosclerotic process.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Adult Endothelial Progenitor Cell, Cell Suspension Culture

DISEASE(S): Atherosclerosis

SUBMITTER: Carmen Duran

LAB HEAD: MªCarmen Duran-Ruiz

PROVIDER: PXD019395 | Pride | 2021-09-09

REPOSITORIES: Pride

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Atherosclerotic Pre-Conditioning Affects the Paracrine Role of Circulating Angiogenic Cells Ex-Vivo.

Eslava-Alcon Sara S Extremera-García Mª Jesús MJ Sanchez-Gomar Ismael I Beltrán-Camacho Lucía L Rosal-Vela Antonio A Muñoz Javier J Ibarz Nuria N Alonso-Piñero Jose Angel JA Rojas-Torres Marta M Jiménez-Palomares Margarita M González-Rovira Almudena A Conejero Rosario R Doiz Esther E Rodriguez-Piñero Manuel M Moreno-Luna Rafael R Durán-Ruiz Mª Carmen MC

International journal of molecular sciences 20200724 15

In atherosclerosis, circulating angiogenic cells (CAC), also known as early endothelial progenitor cells (eEPC), are thought to participate mainly in a paracrine fashion by promoting the recruitment of other cell populations such as late EPC, or endothelial colony-forming cells (ECFC), to the injured areas. There, ECFC replace the damaged endothelium, promoting neovascularization. However, despite their regenerative role, the number and function of EPC are severely affected under pathological co ...[more]

PMID: 32722151

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