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Peptides in plasma, urine, and dialysate: towards unraveling renal peptide handling

ABSTRACT: We investigated the endogenous peptidomes of spent hemodialysate, urine, and plasma, to shed light on peptide handling in the kidney. Our study was based on the hypothesis that hemodialysis replaces glomerular filtration and aims at gaining first insight into the comparative distribution of the peptidome/proteome in these body fluids. These data are expected to support insight into the underlying biological and physiological processes that take place in the kidney, which may help to advance treatment in advanced-stage chronic kidney disease and detoxification in renal replacement therapies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Kidney Failure

SUBMITTER: Martin Pejchinovski

LAB HEAD: Martin Pejchinovski

PROVIDER: PXD019407 | Pride | 2020-07-31

REPOSITORIES: Pride

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			Action	DRS
	Dialysis_sample1.mgf	Mgf
	Dialysis_sample1.msf	Msf
	Dialysis_sample1.raw	Raw
	Dialysis_sample10.mgf	Mgf
	Dialysis_sample10.msf	Msf

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Publications

Peptides in Plasma, Urine, and Dialysate: Toward Unravelling Renal Peptide Handling.

He Tianlin T Pejchinovski Martin M Mullen William W Beige Joachim J Mischak Harald H Jankowski Vera V

Proteomics. Clinical applications 20200723 1

<h4>Purpose</h4>The peptidomes of spent hemodialysate, urine, and plasma are investigated, to shed light on peptide handling in the kidney.<h4>Experimental design</h4>Fifteen plasma, 15 urine, and 13 spent hemodialysate samples are collected from age- and sex-matched subjects with chronic kidney disease. Peptide identification and quantification are performed with capillary electrophoresis-coupled mass spectrometry.<h4>Results</h4>A total of 6278 urinary peptides, 1743 plasma peptides, and 1727 ...[more]

PMID: 32618437

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Arteriovenous Fistulae in Chronic Kidney Disease and the Heart: Physiological, Histological, and Transcriptomic Characterization of a Novel Rat Model

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Renal systems biology of patients with systemic inflammatory response syndrome

Project description:A systems biology approach was used to comprehensively examine the impact of renal disease and hemodialysis (HD) on host response during critical illness. We examined the metabolome, proteome, and transcriptome of 150 patients with critical illness, stratified by renal function. Plasma metabolite values showed greater changes as renal function declined, with the greatest derangements in patients receiving chronic HD. Specifically, 6 uremic retention molecules, 17 other protein catabolites, 7 modified nucleosides, and 7 pentose phosphate sugars increased as renal function declined, consistent with decreased excretion or increased catabolism of amino acids and ribonucleotides. Similarly, the proteome showed increased levels of low-molecular weight proteins and acute phase reactants. The transcriptome revealed a broad-based decrease in mRNA levels among HD patients. Systems integration revealed an unrecognized association between plasma RNASE1 and several RNA catabolites and modified nucleosides. Further, allantoin, N1-methyl-4-pyridone-3-carboxamide, and n-acetylaspartate showed inverse correlations with the majority of significantly down-regulated genes. In conclusion, renal function broadly affected the plasma metabolome, proteome, and peripheral blood transcriptome during critical illness. These changes were not effectively mitigated by hemodialysis. These studies suggest several novel mechanisms whereby renal dysfunction contributes to critical illness. We sequenced peripheral blood RNA of 133 representative subjects with systemic inflammatory response syndrome that had Acute Kidney Injury (AKI) or Hemodialysis (HD). No injury (AKI0; n= 58); AKI Stage 1 (AKI1; n= 36); AKI stage 2 and 3 (AKI23; n= 17); HD (N=22).

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Proteome of HDL from non-diabetic hemodialysis patients

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Microarray-predicted marker genes indicating handling stress in maraena whitefish (Coregonus maraena)

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