Dataset Information

Wheat mutant “RYNO3936” is associated with delayed drought-induced leaf senescence and rapid drought recovery

ABSTRACT: Random mutagenesis was applied to produce a new wheat mutant (RYNO3926) with superior characteristics regarding tolerance to water deficit treatment and rapid recovery from water stress conditions. Under water stress conditions mutant plants reached maturity faster and produced more seeds than its wild type wheat progenitor Further, whereas wild-type Tugela DN plants died within 7 days after induction of water stress, mutant plants survived by maintaining a higher relative moisture content (RMC), increased total chlorophyll, a higher photosynthesis rate and stomatal conductance suggesting that the mutant may possess a “stay green”’ phenotype. Analysis of the proteome of mutant plants revealed that they better regulate post-translational modification (SUMOylation) and have increased expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) proteins. Mutant plants also expressed unique proteins associated with dehydration tolerance including abscisic stress-ripening protein, cold induced protein, cold-responsive protein, dehydrin, Group 3 late embryogenesis and also a lipoprotein (LAlv9) belonging to the family of lipocalins. Overall, our results suggest that our new mutant RYNO3936 has a potential for inclusion in future breeding programs to improve drought tolerance under dryland conditions.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Mixed Disorder As Reaction To Stress

SUBMITTER: Francois Burger

LAB HEAD: Anna-Maria Botha-Oberholster

PROVIDER: PXD019464 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	2018_03_16_AO_RYNO_0_1_180318191853.mzid.gz	Mzid
	2018_03_16_AO_RYNO_0_1_180318191853.mzid.gz_2018_03_16_AO_RYNO_0_1_180318191853.MGF	Mzid
	2018_03_16_AO_RYNO_0_1_180318191853.mzid.gz_2018_03_16_AO_RYNO_0_1_180318191853.pride.mgf.gz	Mzid
	2018_03_16_AO_RYNO_0_1_180318191853.pride.mztab.gz	Mztab
	2018_03_16_AO_RYNO_0_1_180318191853.raw	Raw

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Publications

Wheat Line "RYNO3936" Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery.

le Roux Marlon-Schylor L ML Burger N Francois V NFV Vlok Maré M Kunert Karl J KJ Cullis Christopher A CA Botha Anna-Maria AM

Frontiers in plant science 20200714

Random mutagenesis was applied to produce a new wheat mutant (RYNO3926) with superior characteristics regarding tolerance to water deficit stress induced at late booting stage. The mutant also displays rapid recovery from water stress conditions. Under water stress conditions mutant plants reached maturity faster and produced more seeds than its wild type wheat progenitor. Wild-type Tugela DN plants died within 7 days after induction of water stress induced at late booting stage, while mutant pl ...[more]

PMID: 32760414

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