Dataset Information

Reversible oxidative signaling in Chlamydomonas following nitrogen deprivation

ABSTRACT: As global temperatures climb to historic highs, the far-reaching effects of climate change have impacted agricultural nutrient availability. This has extended to low latitude oceans, where a deficit in both nitrogen and phosphorus stores has led to dramatic decreases in carbon sequestration in oceanic phytoplankton. Although Chlamydomonas reinhardtii, a freshwater model green alga, has shown drastic systems-level alterations following nitrogen deprivation, the mechanism(s) through which these alterations are triggered and regulated are unclear. This study examined the role of reversible oxidative signaling in the nitrogen stress response of C. reinhardtii. Using oxidized cysteine resin-assisted capture enrichment coupled with label-free quantitative proteomics, 7,889 unique oxidized cysteine thiol identifiers were quantified, with 231 significantly changing peptides from 184 proteins following 2 h of nitrogen deprivation. These results demonstrate proteins affected by oxidation involved in nitrogen assimilation, amino acid metabolism, photosynthesis, and other metabolic processes. An enhanced role of non-damaging oxidative pathways is observed throughout the photosynthetic apparatus that provides a framework for further analysis in phototrophs.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Photosynthetic Cell

SUBMITTER: Leslie Hicks

LAB HEAD: Leslie M. Hicks

PROVIDER: PXD019491 | Pride | 2020-06-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200125_ALS_ndep_global_N1.raw	Raw
	20200125_ALS_ndep_global_N2.raw	Raw
	20200125_ALS_ndep_global_N3.raw	Raw
	20200125_ALS_ndep_global_N4.raw	Raw
	20200125_ALS_ndep_global_T1.raw	Raw

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Publications

Photosynthetic Metabolism and Nitrogen Reshuffling Are Regulated by Reversible Cysteine Thiol Oxidation Following Nitrogen Deprivation in Chlamydomonas.

Smythers Amanda L AL McConnell Evan W EW Lewis Hailey C HC Mubarek Saher N SN Hicks Leslie M LM

Plants (Basel, Switzerland) 20200623 6

As global temperatures climb to historic highs, the far-reaching effects of climate change have impacted agricultural nutrient availability. This has extended to low latitude oceans, where a deficit in both nitrogen and phosphorus stores has led to dramatic decreases in carbon sequestration in oceanic phytoplankton. Although <i>Chlamydomonas reinhardtii</i>, a freshwater model green alga, has shown drastic systems-level alterations following nitrogen deprivation, the mechanisms through which the ...[more]

PMID: 32585825

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