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Immunoreactive proteins in the oesophageal gland cells of Anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis

ABSTRACT: Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, while 82 spots in 2D. The corresponding protein bands and spots were excised from the silver stained gel and protein assignation was made by MALDI-TOF/TOF. A total of eleven proteins (including isoforms) were unambiguosly identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Anisakis Simplex Complex

SUBMITTER: Sergio Ciordia

LAB HEAD: Alfonso Navas

PROVIDER: PXD019580 | Pride | 2021-09-09

REPOSITORIES: Pride

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Immunoreactive Proteins in the Esophageal Gland Cells of Anisakis Simplex Sensu Stricto Detected by MALDI-TOF/TOF Analysis.

Robertson Lee L C Arcos Susana S Ciordia Sergio S Carballeda-Sanguiao Noelia N Mena María Del Carmen MDC Sánchez-Alonso Isabel I Gonzalez-Muñoz Miguel M Careche Mercedes M Navas Alfonso A

Genes 20200622 6

In plant and animal nematode parasites, proteins derived from esophageal gland cells have been shown to be important in the host-nematodes relationship but little is known about the allergenic potential of these proteins in the genus <i>Anisakis</i>. Taking into account the increase of anisakiasis and allergies related to these nematodes, immunoreactive properties of gland cell proteins were investigated. Two hundred ventricles were manually dissected from L3 stage larvae of <i>Aniskakis simplex ...[more]

PMID: 32580523

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