Dataset Information

Protein interactions of TMEM147 and TMEM129 proteins from HeLa cells in co-IP experiments

ABSTRACT: IP/MS waw used to identify protein interactions for TMEM147.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Frank Stein

LAB HEAD: Niovi Santama

PROVIDER: PXD019598 | Pride | 2020-08-03

REPOSITORIES: Pride

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Publications

TMEM147 interacts with lamin B receptor, regulates its localization and levels, and affects cholesterol homeostasis.

Christodoulou Andri A Maimaris Giannis G Makrigiorgi Andri A Charidemou Evelina E Lüchtenborg Christian C Ververis Antonis A Ververis Antonis A Georgiou Renos R Lederer Carsten W CW Haffner Christof C Brügger Britta B Santama Niovi N

Journal of cell science 20200821 16

The structurally and functionally complex endoplasmic reticulum (ER) hosts critical processes including lipid synthesis. Here, we focus on the functional characterization of transmembrane protein TMEM147, and report that it localizes at the ER and nuclear envelope in HeLa cells. Silencing of <i>TMEM147</i> drastically reduces the level of lamin B receptor (LBR) at the inner nuclear membrane and results in mistargeting of LBR to the ER. LBR possesses a modular structure and corresponding bifuncti ...[more]

PMID: 32694168

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