Dataset Information

Methyl-SILAF mapping of the Drosophila mitochondrial protein methylome

ABSTRACT: S-adenosylmethionine (SAM) is the principal biological methyl group donor for a diverse range of substrates. It is synthesised in the cytosolic methionine cycle and shuttled throughout the cell. The mitochondrial SAM (mitoSAM) pool depends on import through the inner-membrane S-adenosylmethionine carrier (SAMC) and supports the maturation of metabolites and mitochondrial RNAs. Mutations in SAMC in patients cause a severe metabolic crisis, however, the organellar regulation of mitoSAM and the protein methylation landscape within mitochondria are largely unknown. We developed a fly-compatible SILAC labelling technique and mapped mitochondrial protein methylation sites in Drosophila melanogaster, further showing that SAM is the sole methyl group donor for these modifications, with no contribution from folate-species. This dataset comprises MS-runs used for quality control of methyl-SILAF, the methylome mapping and exclusion of folates as protein methyl-group donors.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Larva

SUBMITTER: Florian Schober

LAB HEAD: Anna Wredenberg

PROVIDER: PXD019654 | Pride | 2021-02-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	170301_QE_IA_NL_FS_Met_01.raw	Raw
	170301_QE_IA_NL_FS_Met_01_02.raw	Raw
	170301_QE_IA_NL_FS_Met_02.raw	Raw
	170301_QE_IA_NL_FS_Met_02_02.raw	Raw
	170301_QE_IA_NL_FS_Met_03.raw	Raw

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Publications

The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters.

Rosenberger Florian A FA Moore David D Atanassov Ilian I Moedas Marco F MF Clemente Paula P Végvári Ákos Á Fissi Najla El NE Filograna Roberta R Bucher Anna-Lena AL Hinze Yvonne Y The Matthew M Hedman Erik E Chernogubova Ekaterina E Begzati Arjana A Wibom Rolf R Jain Mohit M Nilsson Roland R Käll Lukas L Wedell Anna A Freyer Christoph C Wredenberg Anna A

Science advances 20210219 8

Induction of the one-carbon cycle is an early hallmark of mitochondrial dysfunction and cancer metabolism. Vital intermediary steps are localized to mitochondria, but it remains unclear how one-carbon availability connects to mitochondrial function. Here, we show that the one-carbon metabolite and methyl group donor <i>S</i>-adenosylmethionine (SAM) is pivotal for energy metabolism. A gradual decline in mitochondrial SAM (mitoSAM) causes hierarchical defects in fly and mouse, comprising loss of ...[more]

PMID: 33608280

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