Dataset Information

Whole-Genome Wide Quantitative Proteomics Studies Revealed Tissue-Specific Expression and Phosphorylation of Regulatory Proteins in Arabidopsis

ABSTRACT: Organogenesis in plants occurs during all stages of the life cycle. Although previous studies have identified many genes important for either vegetative or reproductive development at RNA level, global scale information of translational and post-translational regulatory levels is still limited. Here, six Arabidopsis stages/organs were analyzed using quantitative proteomics and phosphoproteomics, identifying 2,187 non-redundant proteins and 1,194 phosphoproteins. Compared to that in cauline leaves, expression of 1,445, 1,644, and 1,377 proteins showed greater than 1.5-fold alteration in stage 1-9 flowers, stage 10-12 flowers, and open flowers, respectively. In addition, 294 phosphoproteins with 472 phosphorylation sites were newly discovered, including 275 showing differential expression patterns, providing possible candidates for functional studies or molecular markers. In addition, more phosphorylated proteins were identified in reproductive stages (1,149) than in the vegetative process (995). 701 of the proteins differentially phosphorylated between flowers and leaves were predicted to localize to the nucleus, including 22 transcription factors. Moreover, phosphorylation of CDPK6 and MAPK6 and their interacting proteins, were enriched in reproductive tissues. Overall, our study yielded extensive data on protein expression and phosphorylation at six stages and provides an important resource for understanding regulatory mechanisms during plant development.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Flower, Root, Whole Body, Rosette, Diploid Cell

DISEASE(S): Disease Free

SUBMITTER: Mengyu Li

LAB HEAD: Fang Chang

PROVIDER: PXD019691 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Orbi02901LYH-MH-YJY-T1_151104174746.msf	Msf
	Orbi02901LYH-MH-YJY-T1_151104174746.raw	Raw
	Orbi02902LYH-MH-YJY-T2.msf	Msf
	Orbi02902LYH-MH-YJY-T2.raw	Raw
	Orbi02903LYH-MH-YJY-T3.msf	Msf

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Publications

Global Quantitative Proteomics Studies Revealed Tissue-Preferential Expression and Phosphorylation of Regulatory Proteins in <i>Arabidopsis</i>.

Lu Jianan J Fu Ying Y Li Mengyu M Wang Shuangshuang S Wang Jingya J Yang Qi Q Ye Juanying J Zhang Xumin X Ma Hong H Chang Fang F

International journal of molecular sciences 20200825 17

Organogenesis in plants occurs across all stages of the life cycle. Although previous studies have identified many genes as important for either vegetative or reproductive development at the RNA level, global information on translational and post-translational levels remains limited. In this study, six <i>Arabidopsis</i> stages/organs were analyzed using quantitative proteomics and phosphoproteomics, identifying 2187 non-redundant proteins and evidence for 1194 phosphoproteins. Compared to the e ...[more]

PMID: 32854314

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