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Neuropilin 1 interactome in endothelial cells

ABSTRACT: To get unbiased insights on the molecular networks through by which Neuropilin 1 (NRP1) controls endothelial cell (EC) and blood vessel functions independently from its ligands, wehave implemented a Halo Tag (HT)-based approach, which enables effective surface labeling and pull-down of proteins, and combined it with quantitative mass spectrometry (MS) analysis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endothelial Cell Of Umbilical Vein, Cell Culture

SUBMITTER: Sara Zanivan

LAB HEAD: Sara Zanivan

PROVIDER: PXD019700 | Pride | 2022-06-15

REPOSITORIES: Pride

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			Action	DRS
	20131030_Elite_LiNe_Collab_ingel_neurop_A1.raw	Raw
	20131030_Elite_LiNe_Collab_ingel_neurop_A2.raw	Raw
	20131030_Elite_LiNe_Collab_ingel_neurop_A3.raw	Raw
	20131030_Elite_LiNe_Collab_ingel_neurop_A4.raw	Raw
	20131030_Elite_LiNe_Collab_ingel_neurop_A5.raw	Raw

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Project description:HUVECs transfected with siRNA targeting Neuropilin 1(NRP1) (si-NRP1) or a control non-targeting sequence (si-control) and exposed to unidirectional shear stress (20dyns/cm2), oscillatory flow (5dyn/cm2) or kept in absence of flow.

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