Dataset Information

Snake venom color and LAAO: an evidence of long-term captive Crotalus durissus terrificus venom plasticity

ABSTRACT: The venom color variation of C. d. terrificus (Cdt) is attributed to the presence of the toxin LAAO. However, the driving mechanisms of such variability have not been studied and identified so far. During the venom milking routine at Butantan Institute, we have noticed that most of the venoms of captive Cdt specimens show a yellowish color, while most of the venoms of wild specimens are white. Here we describe a comparative analysis of long-term captive (LTC) and recently wild-caught (RWC) Cdt, focusing on the enzyme LAAO. For the identification of LAAO in individual venoms, four different approaches were employed: evaluation of the enzymatic activity, SDS-PAGE, Western blotting and ELISA. In addition, mass spectrometry analysis was performed using pooled samples. Although some variation among these methodologies was observed, it was clear the significative higher percentage of individual venom samples presenting LAAO in LTC venoms. LAAO was identified in 60-80% LTC specimens and in only 10-12% of RWC specimens. Furthermore, this enzyme accounts for 5.6% of total venom proteins of LTC Cdt pooled venom, while it corresponds to only 0.7% of RWC Cdt pooled venom. These findings strongly suggest that the captive maintenance increases the expression of LAAO in Cdt venom.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Crotalus Durissus Terrificus

TISSUE(S): Venom

SUBMITTER: Alexandre Keiji Tashima

LAB HEAD: Alexandre Keiji Tashima

PROVIDER: PXD019730 | Pride | 2023-10-23

REPOSITORIES: Pride

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			Action	DRS
	20200130_Cdt_Cativeiro_Eduardo_Karen_001.mzML	Mzml
	20200130_Cdt_Cativeiro_Eduardo_Karen_001.raw.rar	Raw
	20200130_Cdt_Cativeiro_Eduardo_Karen_002.mzML	Mzml
	20200130_Cdt_Cativeiro_Eduardo_Karen_002.raw.rar	Raw
	20200130_Cdt_Natureza_Eduardo_Karen_001.mzML	Mzml

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Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity.

Lima Eduardo Oliveira Venancio de EOV Tasima Lídia Jorge LJ Hatakeyama Daniela Miki DM Serino-Silva Caroline C Rodrigues Caroline Fabri Bittencourt CFB Galizio Nathália da Costa NDC Chiarelli Tassia T Nishiduka Erika Sayuri ES Rocha Marisa Maria Teixeira da MMTD Sant'Anna Sávio Stefanini SS Grego Kathleen Fernandes KF Tashima Alexandre Keiji AK Tanaka-Azevedo Anita Mitico AM Morais-Zani Karen de K

Toxicon : official journal of the International Society on Toxinology 20210127

The venom color variation of Crotalus durissus terrificus (Cdt) is attributed to the presence of the toxin L-amino acid oxidase (LAAO). During the venom milking routine of Instituto Butantan, we have noticed that most venoms of captive Cdt specimens show a yellowish color, while most venoms of wild specimens are white. Here we describe a comparative analysis of long-term captive (LTC) and recently wild-caught (RWC) Cdt, focusing on LAAO variation. For the identification of LAAO in individual ven ...[more]

PMID: 33515573

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