Proteomics

Dataset Information

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SmD3 protein interactome in Drosophila melanogaster wing imaginal discs

ABSTRACT: Screening for binding partners of the splicing factor SmD3 and changes in interaction upon depletion of the protein Ecdysoneless (Ecd) in the nubbin domain of third-instar larval wing imaginal discs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster Drosophila Melanogaster (fruit Fly)

TISSUE(S): Wing Disc

SUBMITTER: Steffen Erkelenz  

LAB HEAD: Mirka Uhlirova

PROVIDER: PXD019761 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

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Action DRS
1597_matrix17.xlsx Xlsx
20190122_Q2_UC_ColID_752_ProjID_1597_Uhlirova_01.raw Raw
20190122_Q2_UC_ColID_752_ProjID_1597_Uhlirova_02.raw Raw
20190122_Q2_UC_ColID_752_ProjID_1597_Uhlirova_03.raw Raw
20190122_Q2_UC_ColID_752_ProjID_1597_Uhlirova_04.raw Raw
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Publications

Pre-mRNA splicing catalyzed by the spliceosome represents a critical step in the regulation of gene expression contributing to transcriptome and proteome diversity. The spliceosome consists of five small nuclear ribonucleoprotein particles (snRNPs), the biogenesis of which remains only partially understood. Here we define the evolutionarily conserved protein Ecdysoneless (Ecd) as a critical regulator of U5 snRNP assembly and Prp8 stability. Combining Drosophila genetics with proteomic approaches  ...[more]

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