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Proteomic analysis of mycelial exudate of Ustilaginoidea virens

ABSTRACT: Rice false smut (RFS) is a kind of fungal disease transforming panicles and spikelets into greenish spore balls, caused by Ustilaginoidea virens. During artificial cultivation process, macroscopic exudates could be observed, which is a common feature in many kinds of fungi. We characterized the proteome of exudate associated with this plant pathogen. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was used to identify proteins in the pathogen exudate. A total of 685 proteins comprising 3,949 peptides were identified from the exudate. This study regarded the biological characteristics of U. virens as an entry point. By studying the protein components of the exudates of U. virens, it is helpful to better understand the occurrence and pathogenic mechanism of pathogen and provide a theoretical basis for the control of RFS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ustilaginoidea Virens

SUBMITTER: Songhong Wei

LAB HEAD: Songhong Wei

PROVIDER: PXD019861 | Pride | 2021-04-08

REPOSITORIES: pride

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Proteomic Analysis of Mycelial Exudates of Ustilaginoidea virens.

Wang Haining H Yang Xiaohe X Wei Songhong S Wang Yan Y

Pathogens (Basel, Switzerland) 20210318 3

Rice false smut (RFS) disease, which is caused by Ustilaginoidea virens, has been widespread all over the world in recent years, causing irreversible losses. Under artificial culture conditions, exudates will appear on colonies of U. virens during the growth of the hyphae. Exudation of droplets is a common feature in many fungi, but the functions of exudates are undetermined. As the executors of life functions, proteins can intuitively reflect the functions of exudates. Shotgun pro ...[more]

PMID: 33803797

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