Data-dependent and independent acquisition of Salmonella in epithelial cells throughout the infection process
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ABSTRACT: Viewing the scarce amount of protein material coming from the bacterial pathogen in infection models and despite the availability of contemporary, highly sensitive and fast scanning mass spectrometers, the power requirement still not suffices to study the host and pathogen proteomes simultaneously. In the present work we aimed to establish a DIA mass spectrometry workflow for improving the protein identification and quantification of LC-MS/MS, in Salmonella infected epithelial cells, therefore enabling simultaneous host and pathogen protein expression profiling at different time post infection.
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human) Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. Sl1344
TISSUE(S): Epithelial Cell, Cell Culture
SUBMITTER: Ursula Fels
LAB HEAD: Petra Van Damme
PROVIDER: PXD019863 | Pride | 2023-07-29
REPOSITORIES: Pride
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