Proteomics

Dataset Information

0

Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases


ABSTRACT: Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, a series of glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan oligosaccharides have been synthesised. These compounds are highly efficient inhibitors of HiCel7B, a well-known GH7 endo-β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β-D-glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Aspergillus Niger Nrrl3

SUBMITTER: Bogdan Florea  

LAB HEAD: Bogdan I Florea

PROVIDER: PXD019930 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ABPP_01.zip.raw Raw
ABPP_02.zip.raw Raw
ABPP_03.zip.raw Raw
QIP_searchresults_endo_16db_iproteins.xlsx Xlsx
comp_ABPP_01.zip.raw Raw
Items per page:
1 - 5 of 10
altmetric image

Publications


Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases,  ...[more]

Similar Datasets

2020-03-06 | MSV000085068 | MassIVE
| PRJEB31386 | ENA
| MTR_3g082440 | ExpressionAtlas
| MTR_5g009970 | ExpressionAtlas
| MTR_4g074960 | ExpressionAtlas
| MTR_8g099410 | ExpressionAtlas
| MTR_2g028480 | ExpressionAtlas
2022-09-30 | E-MTAB-12077 | biostudies-arrayexpress
2025-01-10 | GSE286220 | GEO
| PGSC0003DMG400004992 | ExpressionAtlas