Proteomics

Dataset Information

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Digital Microfluidic Isolation of Single Cells for -Omics


ABSTRACT: Proteomic data from 0,1, and 5 U87 cells.A custom single cell proteomic pipeline was developed to collect cell lysates and evaluate them by LC-MS/MS.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jiaxi Peng  

LAB HEAD: Aaron R. Wheeler

PROVIDER: PXD019958 | Pride | 2020-10-26

REPOSITORIES: Pride

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Publications


We introduce Digital microfluidic Isolation of Single Cells for -Omics (DISCO), a platform that allows users to select particular cells of interest from a limited initial sample size and connects single-cell sequencing data to their immunofluorescence-based phenotypes. Specifically, DISCO combines digital microfluidics, laser cell lysis, and artificial intelligence-driven image processing to collect the contents of single cells from heterogeneous populations, followed by analysis of single-cell  ...[more]

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