Proteomic Analysis of Liver in Diet-induced Hyperlipidemic Mice under Fructus Rosa roxburghii action

ABSTRACT: Fructus Rosae Roxburghii (FRR) has been considered as edible and medicinal fruit possessing antiatherosclerotic effect, but the mechanism is still unclear. Hyperlipidemia (HLP) is material basis for atherosclerosis (AS) formation. Under FRR action, TC, TG, LDL, HDL and ASI in serum were regulated to control level. Differentially expressed proteins in liver were analyzed by using TMT labeling and LC-MS/MS for better understanding the effect and molecular mechanism of FRR on diet-induced hyperlipidemic mice. In total, 4460 proteins were quantified, of which 469 proteins showed dramatic changes between each group. According to molecular functions, 25 differentially co-expressed proteins were divided into five categories: substance metabolism, energy transformation and signal transduction, transcription and translation, immune defense. 15 key proteins involved lipids metabolism, which were identified as Cyp7a1, Cyp3a11, Tm7sf2, COAT2, CSAD, RBP3, Lpin1, Dhrs4, Aldh1b1, GK, Acot 4, TSC22D1, PGFS, EHs, GSTM1. Their altered expression suggested that FRR could maintain the metabolism homeostasis by regulating the metabolism of fatty acids, biosynthesis of BAs and steroids, and production of lipid peroxides (LPOs) in mice. It’s first time potential antiatherosclerotic mechanism of FRR regulating blood lipids was explored from protein level, which is of great significance to explore new drug targets for AS.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Pingping Song  

LAB HEAD: Pingping Song

PROVIDER: PXD019962 | Pride | 2021-01-04

REPOSITORIES: Pride