The spatial network of skeletal proteins in a stony coral

ABSTRACT: Coral skeletons are materials composed of inorganic aragonitic fibers, proteins, sugars and lipids that are highly organized to form a solid body upon which the animals live. The skeleton contains more than 30 proteins, all of which are encoded in the animal genome and secreted during the biomineralization process. How these proteins are spatially related is unknown. Using a combination of chemical crosslinking and high-resolution tandem mass spectrometry, we identify, for the first time. the spatial interactions of the skeletal proteins within a stony coral. Our subsequent network analysis revealed several coral acid-rich proteins (CARPs) are invariably associated with carbonic anhydrase(s), alpha-collagen, cadherins and other calcium binding proteins. These interactions clearly show that protein-protein interactions in coral skeletons are highly coordinated and are key to understanding the formation and persistence of coral skeletons through time

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Stylophora Pistillata

TISSUE(S): Tissues

SUBMITTER: Manjula Mummadisetti  

LAB HEAD: Paul G. Falkowski

PROVIDER: PXD020076 | Pride | 2021-01-25

REPOSITORIES: Pride