Chemical Proteomics Tracks Virus Entry and Uncovers NCAM1 As Zika Virus Receptor

ABSTRACT: We present a time-resolved chemical proteomic strategy to track the early-stage entry of ZIKV into host cells. ZIKV was labeled on its surface with a chemical probe, which carries a photocrosslinker to covalently link virus-interacting proteins in living cells on UV exposure at different time points, and a biotin tag for subsequent enrichment and mass spectrometric identification of the receptor or other host proteins critical for virus internalization. We identified Neural Cell Adhesion Molecule (NCAM1) as a potential ZIKV receptor and further validated it through overexpression, knockout, and inhibition of NCAM1 in Vero cells and human glioblastoma cells U-251 MG. Collectively, the strategy can serve as a universal tool to map virus entry pathways and uncover key interacting proteins.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Macaca Mulatta (rhesus Macaque)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Disease Free

SUBMITTER: Ying Zhang  

LAB HEAD: W.Andy Tao

PROVIDER: PXD020119 | Pride | 2020-09-11

REPOSITORIES: Pride