Dataset Information

Creation of a Candida albicans spectral library by Data Dependent Acquisition (DDA)

ABSTRACT: The incidence of candidiasis, caused mainly by the opportunistic fungal Candida albicans, remains a major concern in public health due to the emerging antifungal resistance. C. albicans modulates their proteome under different conditions trying to cope with several stressors in host niches. In this study we developed a C. albicans spectral library by analysing a total of 52 samples corresponding to cells treated with 5 and 10 mM hydrogen peroxide, 40 and 60 mM acetic acid and in the interaction with human macrophages (THP-1) at 3, 6 and 9 h (4 biological replicates). This samples represented several morphologies of C. albicans (yeast and hypha) and different culture conditions (temperature, media…) that contributed to the incorporation of different proteins into the library. The analysis of these conditions by DDA mode allowed the construction of a C. albicans spectral library comprising information of 2879 proteins and 25645 peptides which entailed a 46.5% of the total proteome. The C. albicans spectral library created constitutes a powerful proteomic tool available for the scientific community and could be useful for global (DIA) or targeted (SRM and PRM) studies.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Candida Albicans (yeast)

TISSUE(S): Cell Culture

SUBMITTER: Eduardo Romeu

LAB HEAD: Concha Gil García

PROVIDER: PXD020195 | Pride | 2021-09-30

REPOSITORIES: Pride

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Publications

Extending the Proteomic Characterization of Candida albicans Exposed to Stress and Apoptotic Inducers through Data-Independent Acquisition Mass Spectrometry.

Amador-García Ahinara A Zapico Inés I Borrajo Ana A Malmström Johan J Monteoliva Lucía L Gil Concha C

mSystems 20211005 5

Candida albicans is a commensal fungus that causes systemic infections in immunosuppressed patients. In order to deal with the changing environment during commensalism or infection, C. albicans must reprogram its proteome. Characterizing the stress-induced changes in the proteome that C. albicans uses to survive should be very useful in the development of new antifungal drugs. We studied the C. albicans global proteome after exposure to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and acetic a ...[more]

PMID: 34609160

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	Ahinara_S1703_204.mgf	Mgf
	Ahinara_S1703_204.raw	Raw
	Ahinara_S1703_205.mgf	Mgf
	Ahinara_S1703_205.raw	Raw
	Ahinara_S1703_206.mgf	Mgf