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Quantification of histone H3 and H4 modifications by mass spectrometry in wildtype and MCM2-2A mutants of mice ESCs

ABSTRACT: During DNA replication modified parental histones H3-H4 are segregated almost symmetrically to the two daughter DNA strands enabling mitotic inheritance of histone PTMs. Whether heritable histone modifications confer epigenetic regulation by maintaining chromatin states and gene expression is unclear. Using MCM2 histone-binding mutant embryonic stem cells (ESCs) and mass spectrometry, we show that asymmetric segregation of parental histones to the leading strand causes imbalanced inheritance of histone H3K27 methylations to daughter cells.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Victor Solis

LAB HEAD: Anja Groh

PROVIDER: PXD020326 | Pride | 2023-08-30

REPOSITORIES: Pride

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			Action	DRS
	Peptide_Ratio_Results_041219_filtered.csv	Csv
	Ref_xxx_MVA_AWE_R1_MUT421_081119.raw	Raw
	Ref_xxx_MVA_AWE_R1_MUT439_081119.raw	Raw
	Ref_xxx_MVA_AWE_R1_MUT442_081119.raw	Raw
	Ref_xxx_MVA_AWE_R1_MUT443_081119.raw	Raw

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