Dataset Information

Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks

ABSTRACT: Delineating the extracellular interaction network of the cell surface proteotype (the surfaceome) is fundamental to understanding cellular signaling function in health and disease. Here, we developed LUX-MS, an optoproteomic technology that enables the spatiotemporal and proteome-wide study of surfaceome signaling architectures at nanoscale level without the need for genetic manipulation. A tunable, light-triggered singlet oxygen generator (SOG) based mechanism mediates in-situ proximity-tagging for subsequent mass spectrometry-based identification of acute protein interactions in their native cellular context. We applied LUX-MS to the characterization of surfaceome signaling structures engaged by antibodies, small molecule drugs, biologics and intact bacteriophages across organisms and in complex environments. Cell-type resolved dissection of intercellular communication using LUX-MS thereby revealed the molecular architecture of functional immunological signaling synapses in unprecedented detail. Altogether, LUX-MS enables facebooking of the social protein networks within surfaceome signaling architectures and provides an unprecedented molecular framework for the rational design of biomedical intervention strategies.

INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Listeria Phage A500 Homo Sapiens (human) Escherichia Coli Listeria Monocytogenes Serotype 4b Mus Musculus (mouse)

TISSUE(S): B Cell, Primary Cell, Blood Cell, Cell Suspension Culture, T Cell, Dendritic Cell, Cell Culture

DISEASE(S): Lymphoma

SUBMITTER: Maik Müller

LAB HEAD: Bernd Wollscheid

PROVIDER: PXD020481 | Pride | 2021-06-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	HRP_Antibody_DDA_CD20_Control_r1.raw	Raw
	HRP_Antibody_DDA_CD20_Control_r2.raw	Raw
	HRP_Antibody_DDA_CD20_Control_r3.raw	Raw
	HRP_Antibody_DDA_CD20_LFQ.csv	Csv
	HRP_Antibody_DDA_CD20_Sample_r1.raw	Raw

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Publications

Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks.

Müller Maik M Gräbnitz Fabienne F Barandun Niculò N Shen Yang Y Wendt Fabian F Steiner Sebastian N SN Severin Yannik Y Vetterli Stefan U SU Mondal Milon M Prudent James R JR Hofmann Raphael R van Oostrum Marc M Sarott Roman C RC Nesvizhskii Alexey I AI Carreira Erick M EM Bode Jeffrey W JW Snijder Berend B Robinson John A JA Loessner Martin J MJ Oxenius Annette A Wollscheid Bernd B

Nature communications 20211202 1

The molecular nanoscale organization of the surfaceome is a fundamental regulator of cellular signaling in health and disease. Technologies for mapping the spatial relationships of cell surface receptors and their extracellular signaling synapses would unlock theranostic opportunities to target protein communities and the possibility to engineer extracellular signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables the targeted elucidation of acute protein interactions ...[more]

PMID: 34857745

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