Dataset Information

Mass-spectrometry analysis of the human pineal proteome during night and day and in autism.

ABSTRACT: Using mass spectrometry based proteomics and dedicated analysis tools, we identify proteins expressed in the human pineal gland and analyze systematically their variation with time of the throughout the day, and, compare these changes in the pineal proteome between control specimens and donors diagnosed with ASD.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Basis Pedunculi Cerebri, Interstitial Cell Of Pineal Gland

DISEASE(S): Autism Spectrum Disorder

SUBMITTER: MARIETTE MATONDO

LAB HEAD: MARIETTE MATONDO

PROVIDER: PXD020501 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20131209_Q_CB_Bourgeron_04057.raw	Raw
	20131209_Q_CB_Bourgeron_04057b.raw	Raw
	20131209_Q_CB_Bourgeron_09604.raw	Raw
	20131209_Q_CB_Bourgeron_09604b.raw	Raw
	20131209_Q_CB_Bourgeron_09604b_131223030713.raw	Raw

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Publications

Mass-spectrometry analysis of the human pineal proteome during night and day and in autism.

Dumas Guillaume G Goubran-Botros Hany H Matondo Mariette M Pagan Cécile C Boulègue Cyril C Chaze Thibault T Chamot-Rooke Julia J Maronde Erik E Bourgeron Thomas T

Journal of pineal research 20210111 3

The human pineal gland regulates day-night dynamics of multiple physiological processes, especially through the secretion of melatonin. Using mass-spectrometry-based proteomics and dedicated analysis tools, we identify proteins in the human pineal gland and analyze systematically their variation throughout the day and compare these changes in the pineal proteome between control specimens and donors diagnosed with autism. Results reveal diverse regulated clusters of proteins with, among others, c ...[more]

PMID: 33368564

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Project description:Extracellular vesicles (EVs) are membranous particles released by different organisms. EVs carry several kinds of macromolecules implicated in cell communication. EVs have become an important topic in the study of pathogenic fungi, due to their relation with the fungal-host interactions. One of the essential research areas in this field is the characterization protein profile of EV cargo, because of plant fungal pathogens rely heavily on secreted proteins to invade their hosts. However, little is known about EVs of Botrytis cinerea, which is one of the most devastating phytopathogenic fungi. The present study has two main objectives: the first isolation and characterization of B. cinerea EVs; and the description of their potential role during the infective process. All the experimental process was conducted in B. cinerea growing in mimimal salt medium supplemented with glucose as a constitutive stage, and deproteinized tomato cell walls (TCW) as virulence inductor. The isolation of EV was performed by differential centrifugation, filtration, ultrafiltration, and sucrose cushion ultracentrifugation. EV fractions were visualised by TEM using negative staining. Proteomic analysis of EV cargo was addressed by LC-MS/MS. The used methodology had allowed, for the first time, the correct isolation of B. cinerea EVs, and the identification of a high number of EV proteins, including potential EV markers. The isolated EVs displayed differences in the morphology under both assayed conditions. GO analysis of EV proteins showed an enrichment in cell wall metabolism and proteolysis under TCW. KEGG analysis also showed difference in EV function under both conditions, highlighting the presence of potential virulence/pathogenic factors implicated in cell wall metabolism, between others. This is the first evidence and characterization of B. cinerea EVs production, showing that EVs are essential in the infection process of B. cinerea and share crucial functions with the conventional secretion pathway.

Dataset Information

Mass-spectrometry analysis of the human pineal proteome during night and day and in autism.

Dataset's files

Publications

Mass-spectrometry analysis of the human pineal proteome during night and day and in autism.

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