Dataset Information

PASEF technology on timsTOF Pro improves quality of FPOP analysis

ABSTRACT: Fast photochemical oxidation of proteins (FPOP) is a modern technique for studying protein folding, conformations, interactions, etc. In this work, timsTOF Pro mass spectrometer coupled with trapped ion mobility spectrometry was used for identification of oxidative modifications in a model protein. Multiple modifications on the same residues, including six modifications of histidine, were successfully resolved. Moreover, PASEF technology allows successful sequencing even minor populations of modified peptides.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Dmitry Loginov

LAB HEAD: Petr Novak

PROVIDER: PXD020509 | Pride | 2021-06-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20ng_FPOP_20ng_FPOP_laser_protein-peptides.csv	Csv
	20ng_FPOP_Bruker_no_laser_protein-peptides.csv	Csv
	40ng_FPOP_Bruker_laser_protein-peptides.csv	Csv
	40ng_FPOP_Bruker_no_laser_protein-peptides.csv	Csv
	Hpt.fasta	Fasta

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Publications

Benefits of Ion Mobility Separation and Parallel Accumulation-Serial Fragmentation Technology on timsTOF Pro for the Needs of Fast Photochemical Oxidation of Protein Analysis.

Loginov Dmitry S DS Fiala Jan J Chmelik Josef J Brechlin Peter P Kruppa Gary G Novak Petr P

ACS omega 20210408 15

Fast photochemical oxidation of proteins (FPOP) is a recently developed technique for studying protein folding, conformations, interactions, etc. In this method, hydroxyl radicals, usually generated by KrF laser photolysis of H<sub>2</sub>O<sub>2</sub>, are used for irreversible labeling of solvent-exposed side chains of amino acids. Mapping of the oxidized residues to the protein's structure requires pinpointing of modifications using a bottom-up proteomic approach. In this work, a quadrupole t ...[more]

PMID: 34056188

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