Proteomics

Dataset Information

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Combining label-free and label-based accurate quantifications with SWATH-MS: comparison with SRM and PRM for the evaluation of bovine muscle type effects


ABSTRACT: The performances of label-based SWATH-MS, SRM and PRM to accurately quantify ten candidate biomarkers of beef meat tenderness or marbling, in a cohort of 64 bovine muscle tissues expected to cover a wide biological range of these traits, were evaluated. Limits of quantification, dynamic range and quantification performances were assessed. Moreover, protein amounts for all proteins detected in SWATH-MS were estimated in a label-free manner.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Muscle

SUBMITTER: Joanna Bons  

LAB HEAD: Christine Carapito

PROVIDER: PXD020638 | Pride | 2021-06-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BosTaurusiRT_DCpUN_BJ_20171206.fasta Fasta
F051328.dat Other
F053256.dat Other
F053257.dat Other
F053258.dat Other
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Publications

Combining label-free and label-based accurate quantifications with SWATH-MS: Comparison with SRM and PRM for the evaluation of bovine muscle type effects.

Bons Joanna J   Husson Gauthier G   Chion Marie M   Bonnet Muriel M   Maumy-Bertrand Myriam M   Delalande François F   Cianférani Sarah S   Bertrand Frédéric F   Picard Brigitte B   Carapito Christine C  

Proteomics 20210330 10


Mass spectrometry has proven to be a valuable tool for the accurate quantification of proteins. In this study, the performances of three targeted approaches, namely selected reaction monitoring (SRM), parallel reaction monitoring (PRM) and sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS), to accurately quantify ten potential biomarkers of beef meat tenderness or marbling in a cohort of 64 muscle samples were evaluated. So as to get the most benefit out of t  ...[more]

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