Dataset Information

Identification of new proteins related with cisplatin resistance in S. cerevisiae

ABSTRACT: The aim of this study is to select a cisplatin resistant Saccharomyces cerevisiae strain as a model organism to look for new molecular markers of cisplatin resistance and the identification of mechanisms/interactions involved. A cisplatin resistant S. cerevisiae strain was obtained after continuous exposure to the drug during 80 days. Then, total protein extraction, purification and identification were carried out, in wild type (wt) and resistant strains, by tandem mass spectrometry using a "nano HPLC-ESI-MS/MS" ion trap system. The increase in emPAI (resistant vs wt strains) was calculated to study the increase in protein expression. "Genemania" software (http://www.Genemania.org/) was used to compare the effects, functions and interactions of proteins. The selected cisplatin resistant strain showed 2.5 times more resistance than the wt strain (for the ID50 value) and 2.78 times more resistant for the ID90 value. The long-term exposure to cisplatin induced resistance in S. cerevisiae, obtaining an increased expression of QCR2, QCR1, ALDH4, ATPB, ATPA, SCW10, HSP26, ATPG, and PCKA proteins. The overexpression of the above-mentioned proteins suggests that they could be involved in cisplatin resistance. The resistance acquisition process is complex and involves the activation of multiple mechanisms that interact together.

INSTRUMENT(S): DB source UniProt, Mascot, Bruker Daltonics amaZon series, ProteinScape, emPAI value, DB source NCBI

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Miguel Ruiz-Gomez

LAB HEAD: Miguel J. Ruiz-Gómez

PROVIDER: PXD020665 | Pride | 2021-02-25

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	1-WTreportbuilder-MASCOT.csv	Csv
	2-WTProteinTable.xls	Xls
	3-RCisPtreportbuilder-MASCOT.csv	Csv
	4-RCisPtProteinTable.xls	Xls
	5-CommonproteinsinWTandRCisPt.xls	Xls

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Publications

Identification of new proteins related with cisplatin resistance in Saccharomyces cerevisiae.

Burgos-Molina Antonio M AM Mercado-Sáenz Silvia S Cárdenas Casimiro C López-Díaz Beatriz B Sendra-Portero Francisco F Ruiz-Gómez Miguel J MJ

Applied microbiology and biotechnology 20210212 5

The aim of this study is to select a cisplatin-resistant Saccharomyces cerevisiae strain to look for new molecular markers of resistance and the identification of mechanisms/interactions involved. A resistant strain was obtained after 80 days of cisplatin exposure. Then, total protein extraction, purification, and identification were carried out, in wild-type (wt) and resistant strains, by tandem mass spectrometry using a "nano HPLC-ESI-MS/MS" ion trap system. The increase in the exponentially m ...[more]

PMID: 33576883

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