Dataset Information

Single cell glycosylation analysis (ScSUGAR-seq)

ABSTRACT: Proteomic data of ScSUGAR-seq samples: 1)TMT experiment to confirm changes in glycosylation due to Swainsonine 2)LFQ biotin pull down experiment to assess lectin binding partners 3)LFQ of MGAT1 cell lines assessing glycopeptide and glycoproteome level changes

INSTRUMENT(S): Orbitrap Fusion, LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Nichollas Scott

LAB HEAD: Professor Ricky Johnstone

PROVIDER: PXD020685 | Pride | 2021-06-08

REPOSITORIES: Pride

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Publications

SUGAR-seq enables simultaneous detection of glycans, epitopes, and the transcriptome in single cells.

Kearney Conor J CJ Vervoort Stephin J SJ Ramsbottom Kelly M KM Todorovski Izabela I Lelliott Emily J EJ Zethoven Magnus M Pijpers Lizzy L Martin Ben P BP Semple Timothy T Martelotto Luciano L Trapani Joseph A JA Parish Ian A IA Scott Nichollas E NE Oliaro Jane J Johnstone Ricky W RW

Science advances 20210219 8

Multimodal single-cell RNA sequencing enables the precise mapping of transcriptional and phenotypic features of cellular differentiation states but does not allow for simultaneous integration of critical posttranslational modification data. Here, we describe SUrface-protein Glycan And RNA-seq (SUGAR-seq), a method that enables detection and analysis of N-linked glycosylation, extracellular epitopes, and the transcriptome at the single-cell level. Integrated SUGAR-seq and glycoproteome analysis i ...[more]

PMID: 33608275

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			Action	DRS
	Glycopeptide_Byonic_searches.zip	Other
	IP_Input_and_script.zip	Other
	IP_LFQ.zip	Other
	IP_input_LFQ.zip	Other
	LFQ_input_and_scripts.zip	Other