Proteomics

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Quantitative LC-MS/MS Proteomics of human skin and LabSkin model

ABSTRACT: We report for the first time label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in six human skin explants and a 3D living skin equivalent model from LabSkin. More than 2000 proteins were identified and quantified from total cellular protein. This systematic determination of functional equivalence between human skin and LabSkin is a key step towards the construction of a representative human in vitro skin model, which can be used as an alternative to current animal-based tests for chemical safety and for predicting dosage of topically administered drugs.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

SUBMITTER: Jill Barber  

LAB HEAD: Jill Barber

PROVIDER: PXD020742 | Pride | 2021-09-08

REPOSITORIES: Pride

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20160520_NC_C07.raw Raw
20160520_NC_C207.raw Raw
20160520_NC_C252.raw Raw
20160520_NC_C256.raw Raw
20160520_NC_C257.raw Raw
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Publications

ABC transporters (ATP-binding cassette transporter) traffic drugs and their metabolites across membranes, making ABC transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains challenging because they are large and low-abundance transmembrane proteins. Here, we analysed 200 samples of crude and membrane-enriched fractions from human liver, kidney, intestine, brain microvessels and skin, by  ...[more]

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