Dataset Information

Neuron-Derived Exosomes as a tool for the study of Parkinson disease: a rapid workflow for morphology, miRNA and proteomic characterization.Neuron-Derived Exosomes as a tool for the study of Parkinson disease: a rapid workflow for morphology, miRNA and proteomic characterization.Neuron-Derived Exosomes as a tool for the study of Parkinson disease: a rapid workflow for morphology, miRNA and proteomic characterization.

ABSTRACT: Neuron derived exosomes have been enriched from plasma using L1CAM antigen. Vesicles were enriched from plasma of Parkinson Disease patients and controls. The NDE morphology was observed by transmission electron microscope (TEM). miRNA-155 content was studied both in PD-NDE and control-NDE. LC-MS/MS was applied to verify the reproducibility of the NDE enrichment procedure and to assay PD-NDE protein profiles.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Parkinson's Disease

SUBMITTER: Federica Anastasi

LAB HEAD: Liam A. McDonnell

PROVIDER: PXD020794 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	BSAContaminants.fasta	Fasta
	FA20191031_Strap_Brain_exo_PD_MR_multiconsensus_total.pep.xml	Pepxml
	FA20191031_Strap_Brain_exo_PD_MR_multiconsensus_total.prot.xml	Xml
	Multiconsensus_L1CAM_Exo_PD_study.msf	Msf
	OR1_20191030_ES803_10786071_35C_LTrap_FA_Strap_Brain_exo_PD2.raw	Raw

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Publications

Proteomics Profiling of Neuron-Derived Small Extracellular Vesicles from Human Plasma: Enabling Single-Subject Analysis.

Anastasi Federica F Masciandaro Silvia Maria SM Carratore Renata Del RD Dell'Anno Maria Teresa MT Signore Giovanni G Falleni Alessandra A McDonnell Liam A LA Bongioanni Paolo P

International journal of molecular sciences 20210314 6

Small extracellular vesicles have been intensively studied as a source of biomarkers in neurodegenerative disorders. The possibility to isolate neuron-derived small extracellular vesicles (NDsEV) from blood represents a potential window into brain pathological processes. To date, the absence of sensitive NDsEV isolation and full proteome characterization methods has meant their protein content has been underexplored, particularly for individual patients. Here, we report a rapid method based on a ...[more]

PMID: 33799461

Publication: 1/2

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Publications

Proteomics Profiling of Neuron-Derived Small Extracellular Vesicles from Human Plasma: Enabling Single-Subject Analysis.

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