Dataset Information

TMT-based proteomics analysis of mouse germ cells

ABSTRACT: The proteome changes were quantified in RibosomeRPL39L-/- spermatocytes and elongated spermatids using TMT 6-plex, and in spermatogonia, spermatocytes, round spermatids and elongated spermatids using TMT 10-plex by LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos, LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Xuejiang Guo

LAB HEAD: Xuejiang Guo

PROVIDER: PXD020874 | Pride | 2022-10-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Mouse-Rpl39l-tetraploid-100ug-HP-RP-1-20180929.raw	Raw
	Mouse-Rpl39l-tetraploid-100ug-HP-RP-10-20181002.raw	Raw
	Mouse-Rpl39l-tetraploid-100ug-HP-RP-11-20181002.raw	Raw
	Mouse-Rpl39l-tetraploid-100ug-HP-RP-12-20181002.raw	Raw
	Mouse-Rpl39l-tetraploid-100ug-HP-RP-13-20181002.raw	Raw

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Publications

A male germ-cell-specific ribosome controls male fertility.

Li Huiling H Huo Yangao Y He Xi X Yao Liping L Zhang Hao H Cui Yiqiang Y Xiao Huijuan H Xie Wenxiu W Zhang Dejiu D Wang Yue Y Zhang Shu S Tu Haixia H Cheng Yiwei Y Guo Yueshuai Y Cao Xintao X Zhu Yunfei Y Jiang Tao T Guo Xuejiang X Qin Yan Y Sha Jiahao J

Nature 20221214 7941

Ribosomes are highly sophisticated translation machines that have been demonstrated to be heterogeneous in the regulation of protein synthesis1,2. Male germ cell development involves complex translational regulation during sperm formation3. However, it remains unclear whether translation during sperm formation is performed by a specific ribosome. Here we report a ribosome with a specialized nascent polypeptide exit tunnel, RibosomeST, that is assembled with the m ...[more]

PMID: 36517592

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