Dataset Information

Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation

ABSTRACT: Human periodontal ligament stem cells (hPDLSCs) are ideal seed cells for periodontal regeneration. To better understand the dynamic protein profiles during hPDLSCs osteogenic differentiation, we conducted a quantitative proteomics to reveal the temporal protein expression pattern during this process. Clustering and functional annotation by Gene Ontology terms identified distinctions among proteins with different dynamic tendencies. Pathway enrichment analysis with the Kyoto Encyclopedia of Genes and Genomes database found that oxidative phosphorylation (OXPHOS) was significantly involved, which further predicted continuous activation by Ingenuity Pathway Analysis software. Redox-sensitive signalling pathways with dynamic activation status showed associations with OXPHOS to various degrees, especially the sirtuin signalling pathway. SOD2, an important component of the sirtuin signalling pathway, is a hub protein of the interaction network and displays a persistent increase during osteogenesis. Our in-depth dynamic proteomic analysis indicated the comprehensive and dynamic regulation mechanism during hPDLSC osteogenesis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture, Periodontal Ligament

SUBMITTER: Jianjia Li

LAB HEAD: Wei Qiu

PROVIDER: PXD020908 | Pride | 2021-02-11

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	HF_20191130_20cm_60min_onecolumn_TMT_2F1.raw	Raw
	HF_20191130_20cm_60min_onecolumn_TMT_2F10.raw	Raw
	HF_20191130_20cm_60min_onecolumn_TMT_2F2.raw	Raw
	HF_20191130_20cm_60min_onecolumn_TMT_2F3.raw	Raw
	HF_20191130_20cm_60min_onecolumn_TMT_2F4.raw	Raw

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Publications

Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation.

Li Jianjia J Wang Zhifa Z Huang Xiangyu X Wang Zhaodan Z Chen Zehao Z Wang Runting R Chen Zhao Z Liu Wei W Wu Buling B Fang Fuchun F Qiu Wei W

Stem cell research & therapy 20210203 1

<h4>Background</h4>Human periodontal ligament stem cells (hPDLSCs) are ideal seed cells for periodontal regeneration. A greater understanding of the dynamic protein profiles during osteogenic differentiation contributed to the improvement of periodontal regeneration tissue engineering.<h4>Methods</h4>Tandem Mass Tag quantitative proteomics was utilized to reveal the temporal protein expression pattern during osteogenic differentiation of hPDLSCs on days 0, 3, 7 and 14. Differentially expressed p ...[more]

PMID: 33536073

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