Proteomics

Dataset Information

0

Citrus sinensis challenged with LotP


ABSTRACT: Infiltrating purified LotP protein in healthy citrus leaf induces chlorosis and provoke plant stress. Further proteomic analysis of the affected tissue using TMT11MS/MS revealed a significant abundance of plant proteins associated with stabilizing and processing mRNA transcripts and miRNAs. Interestingly, a subset of transcription factors directly connected to pathways coordinating the innate plant defense were also more abundant in the damage tissue two days post infiltration. Inhibiting the formation of LotP multimers via direct mutation of critical residues of the N terminal domain significantly decreases the chlorotic response in infiltrated leaf as well as the intensity of the associated proteome reaction.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Citrus Sinensis (sweet Orange) (citrus Aurantium Var. Sinensis)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Kaylie Padgett-Pagliai  

LAB HEAD: Claudio Fabricio Gonzalez

PROVIDER: PXD020912 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
PM528-TMT1-MMTS-1.fasta Fasta
PM528-TMT1-MMTS-1.msf Msf
PM528-TMT1-MMTS-1.mzML Mzml
PM528-TMT1-MMTS-1.mzid.gz Mzid
Set2.rar Other
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Publications

'<i>Candidatus</i> Liberibacter asiaticus' Multimeric LotP Mediates <i>Citrus sinensis</i> Defense Response Activation.

Merli Marcelo L ML   Padgett-Pagliai Kaylie A KA   Cuaycal Alexandra E AE   Garcia Lucila L   Marano Maria Rosa MR   Lorca Graciela L GL   Gonzalez Claudio F CF  

Frontiers in microbiology 20210804


'<i>Candidatus</i> Liberibacter asiaticus' is known as the most pathogenic organism associated with citrus greening disease. Since its publicized emergence in Florida in 2005, '<i>Ca</i>. L. asiaticus' remains unculturable. Currently, a limited number of potential disease effectors have been identified through <i>in silico</i> analysis. Therefore, these potential effectors remain poorly characterized and do not fully explain the complexity of symptoms observed in citrus trees infected with '<i>C  ...[more]

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