Dataset Information

Comprehensive Proteomic Analysis of Exosomes Derived from Human Bone Marrow-, Adipose Tissue-, and Human Umbilical Cord- Mesenchymal Stem Cells

ABSTRACT: Mesenchymal stem cells (MSCs)-derived exosomes (exo) have shown comprehensive application prospects over the years. Despite similar functions, exomes from different origins present heterogeneous characteristics and components; however, there are no relevant proteomic analyses. In this study, we isolated exosomes from MSCs, derived from different tissues, by ultracentrifugation. A total of 1014 proteins were detected using a label-free method and analyzed with bioinformatics tools. The results revealed their shared function in the extracellular matrix receptor. Bone marrow-MSCs-derived exosomes showed superior regeneration ability. Likewise, adipose tissue-MSCs-derived exosomes played a significant role in immune regulation. Whereas, umbilical cord-MSCs-derived exosomes were more prominent in tissue damage repair.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Zhenggang Wang

LAB HEAD: Zheng-gang Wang

PROVIDER: PXD020948 | Pride | 2021-04-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Z9327LQ_A_MSC_1_Slot2-11_1_2288.d.zip	Other
	Z9327LQ_A_MSC_2_Slot2-11_1_2289.d.zip	Other
	Z9327LQ_A_MSC_3_Slot2-11_1_2290.d.zip	Other
	Z9327LQ_B_MSC_1_Slot2-12_1_2292.d.zip	Other
	Z9327LQ_B_MSC_2_Slot2-12_1_2293.d.zip	Other

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Publications

Comprehensive proteomic analysis of exosomes derived from human bone marrow, adipose tissue, and umbilical cord mesenchymal stem cells.

Wang Zheng-Gang ZG He Zhi-Yi ZY Liang Shuang S Yang Qing Q Cheng Peng P Chen An-Min AM

Stem cell research & therapy 20201127 1

<h4>Background</h4>Mesenchymal stem cell (MSC)-derived exosomes have shown comprehensive application prospects over the years. Despite performing similar functions, exosomes from different origins present heterogeneous characteristics and components; however, the relative study remains scarce. Lacking of a valuable reference, researchers select source cells for exosome studies mainly based on accessibility and personal preference.<h4>Methods</h4>In this study, exosomes secreted by MSCs derived f ...[more]

PMID: 33246507

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