Proteomics

Dataset Information

0

SILAC analysis of exosome secretion in response to centrosome amplification


ABSTRACT: We performed quantitative proteomics analysis by Stable Isotope Labelling by Amino Acids in Cell culture (SILAC) to understand how centrosome amplification changes the composition of human small extracellular vesicles. We carried out SILAC labelling with medium and heavy isotopes, as it enables the exclusion of contaminant serum proteins, which would be unlabeled (equivalent of light labeling), as well as allowing for simultaneous processing of purification steps to decrease sample-to-sample variability. We isolated small extracellular vesicles by ultracentifugation followed by size exclusion chromatography (SEC). All experiments were performed in duplicate with switched SILAC labelling.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Pancreatic Ductal Carcinoma

SUBMITTER: Faraz Mardakheh  

LAB HEAD: Faraz Mardakheh

PROVIDER: PXD020984 | Pride | 2021-01-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
QE1_sophie_7HminusMplus.raw Raw
QE1_sophie_7HplusMinus.raw Raw
QE1_sophie_8HminusMplus.raw Raw
QE1_sophie_8HplusMinus.raw Raw
QE1_sophie_9HminusMplus.raw Raw
Items per page:
1 - 5 of 10

Similar Datasets

2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress
2015-03-06 | E-GEOD-59792 | biostudies-arrayexpress
2011-04-22 | E-GEOD-28813 | biostudies-arrayexpress
2021-07-02 | PXD025169 | Pride
2020-05-15 | PXD013809 | Pride
2022-12-12 | PXD038725 | Pride
2018-11-07 | PXD006900 | Pride
2018-11-07 | PXD006996 | Pride
2019-12-11 | PXD014565 | Pride
2019-02-15 | PXD010438 | Pride