Proteomics

Dataset Information

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PRL-1 selectively dephosphorylates TIMELESS


ABSTRACT: The timing of behavior under natural light-dark conditions is a function of circadian clocks and photic input pathways. Yet a mechanistic understanding of how these pathways collaborate in animals is lacking. Here we demonstrate in Drosophila that the Phosphatase of Regenerating Liver-1 (PRL-1) sets period length and behavioral phase gated by photic signals. PRL-1 knockdown in PDF clock neurons dramatically lengthens circadian period. PRL-1 mutants exhibit allele-specific interactions with the light- and clock-regulated gene timeless (tim). Moreover, we show that PRL-1 promotes TIM accumulation and dephosphorylation. Interestingly, the PRL-1 mutant period lengthening is suppressed in constant light and PRL-1 mutants display a delayed phase under short, but not long, photoperiod conditions. Thus, our studies reveal that PRL-1 dependent dephosphorylation of TIM is a core mechanism of the clock that sets period length and phase in darkness, enabling the behavioral adjustment to changing day-night cycles.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Bridget Lear  

LAB HEAD: Ravi Allada

PROVIDER: PXD020986 | Pride | 2020-11-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
04022013_EK_RA_01.msf Msf
04022013_EK_RA_01.raw Raw
04022013_EK_RA_05.raw Raw
04022013_EK_RA_05_130418152115.msf Msf
04092013_EK_RA_01.msf Msf
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Publications

Phosphatase of Regenerating Liver-1 Selectively Times Circadian Behavior in Darkness via Function in PDF Neurons and Dephosphorylation of TIMELESS.

Kula-Eversole Elżbieta E   Lee Da Hyun DH   Samba Ima I   Yildirim Evrim E   Levine Daniel C DC   Hong Hee-Kyung HK   Lear Bridget C BC   Bass Joseph J   Rosbash Michael M   Allada Ravi R  

Current biology : CB 20201105 1


The timing of behavior under natural light-dark conditions is a function of circadian clocks and photic input pathways, but a mechanistic understanding of how these pathways collaborate in animals is lacking. Here we demonstrate in Drosophila that the Phosphatase of Regenerating Liver-1 (PRL-1) sets period length and behavioral phase gated by photic signals. PRL-1 knockdown in PDF clock neurons dramatically lengthens circadian period. PRL-1 mutants exhibit allele-specific interactions with the l  ...[more]

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