Dataset Information

Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary

ABSTRACT: We describe a method to identify neuropeptides in specific cell-types by the conditional disruption of the gene for carboxypeptidase E (Cpe), an essential neuropeptide-processing enzyme. The loss of Cpe led to the accumulation of neuropeptides containing C-terminal basic residues which are used as tags for affinity-purification followed by identification using quantitative peptidomics. This approach enables the identification of specific forms of neuropeptides in genetically defined populations of cells.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Alexandre Keiji Tashima

LAB HEAD: Alexandre Keiji Tashima

PROVIDER: PXD020999 | Pride | 2020-11-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20181101_Lloyd_LF12_DDA_85min_001.mgf	Mgf
	20181101_Lloyd_LF12_DDA_85min_001.raw.zip	Raw
	20181101_Lloyd_LF14_DDA_85min_001.mgf	Mgf
	20181101_Lloyd_LF14_DDA_85min_001.raw.zip	Raw
	20181129_Lloyd_LF11_DDA_65min_001.mgf	Mgf

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Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary.

Fricker Lloyd D LD Tashima Alexandre K AK Fakira Amanda K AK Hochgeschwender Ute U Wetsel William C WC Devi Lakshmi A LA

Cell chemical biology 20201119 1

Neuropeptides and peptide hormones are important cell-cell signaling molecules that mediate many physiological processes. Unlike classic neurotransmitters, peptides undergo cell-type-specific post-translational modifications that affect their biological activity. To enable the identification of the peptide repertoire of a genetically defined cell type, we generated mice with a conditional disruption of the gene for carboxypeptidase E (Cpe), an essential neuropeptide-processing enzyme. The loss o ...[more]

PMID: 33217339

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