Dataset Information

Phomopsis amygdala fusicoccadiene synthase

ABSTRACT: Fusicoccadiene synthase from Phomopsis amygdali (PaFS) catalyzes the first committed step in the biosynthesis of Fusicoccin A, a diterpene glycoside and a a potential therapeutic agent. PaFS contains a C-terminal prenyltransferase domain that generates geranylgeranyl diphosphate (GGPP) and an N-terminal cyclase domain that utilizes GGPP to generate fusicoccadiene, which comprises the complex 5-8-5 tricyclic hydrocarbon skeleton of Fusicoccin A. The C-termimal domain octamerizes to form a central core, with the eight cyclase domains radiating outward via flexible linker segments with variable extended conformations. Crosslinking-mass spectrometry (XL-MS) experiments affirm and reveal inter-domain interactions, showing that compact conformations can be achieved. These studies, complemented by cryo-EM and functional studies, provide a framework for understanding substrate channeling between consecutive catalytic domains.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Phomopsis Sp. Amygdali Ny7157a

SUBMITTER: Hee Jong Kim

LAB HEAD: benjamin aaron garcia

PROVIDER: PXD021007 | Pride | 2021-07-07

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20191102-hjk-jacque-Pafs-0pt5mgml-DSBU-A10.raw	Raw
	20191102-hjk-jacque-Pafs-0pt5mgml-DSBU-A10_20191103103812.raw	Raw
	20191102-hjk-jacque-Pafs-0pt5mgml-DSBU-A11.raw	Raw
	20191102-hjk-jacque-Pafs-0pt5mgml-DSBU-A11_20191103122619.raw	Raw
	20191102-hjk-jacque-Pafs-0pt5mgml-DSBU-A12.raw	Raw

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Publications

Structural insight on assembly-line catalysis in terpene biosynthesis.

Faylo Jacque L JL van Eeuwen Trevor T Kim Hee Jong HJ Gorbea Colón Jose J JJ Garcia Benjamin A BA Murakami Kenji K Christianson David W DW

Nature communications 20210609 1

Fusicoccadiene synthase from Phomopsis amygdali (PaFS) is a unique bifunctional terpenoid synthase that catalyzes the first two steps in the biosynthesis of the diterpene glycoside Fusicoccin A, a mediator of 14-3-3 protein interactions. The prenyltransferase domain of PaFS generates geranylgeranyl diphosphate, which the cyclase domain then utilizes to generate fusicoccadiene, the tricyclic hydrocarbon skeleton of Fusicoccin A. Here, we use cryo-electron microscopy to show that the structure of ...[more]

PMID: 34108468

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